Isolates of Meloidogyne hapla with distinct mitchondrial genomes

Because two conflicting reports of the structure of the Meloidogyne hapla mitochondrial genome exist, we compared the mitochondrial DNA (mtDNA) purified from two isolates of M. hapla: one from San Bernardino County in southern California (BRDO) and the other from England. The authenticity of the BRD...

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Bibliographic Details
Published in:Journal of nematology 1993-06, Vol.25 (2)
Main Authors: Peloquin, J.J. (University of California, Riverside, CA), Bird, D.M, Kaloshian, I, Matthews, W.C
Format: Article
Language:English
Subjects:
ADN
FILOGENIA
GENOMAS
GENOME
MELOIDOGYNE HAPLA
MITOCHONDRIE
MITOCONDRIA
PHYLOGENIE
POLIMORFISMO
POLYMORPHISME
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Summary:Because two conflicting reports of the structure of the Meloidogyne hapla mitochondrial genome exist, we compared the mitochondrial DNA (mtDNA) purified from two isolates of M. hapla: one from San Bernardino County in southern California (BRDO) and the other from England. The authenticity of the BRDO isolate in particular was confirmed by examination of morphological characters, isoenzyme analysis, and differential host range tests. Restriction analysis revealed that mtDNA from the BRDO and English isolates corresponded to only the structure first reported, although significant differences between the two isolates were apparent. Southern blots probed with cloned, cytochrome oxidase I (cox-1) DNA from Romanomermis culicivorax mtDNA confirmed that the analyzed DNA was of mitochondrial origin. Thus, M. hapla has at least two distinct but presumably related mitchondrial genomes, plus at least one very different structure. These data are discussed with reference to recent molecular diagnostic and phylogenetic analyses of Meloidogyne
ISSN:0022-300X
2640-396X