Resistance of cereals to aphids: the interaction between hydroxamic acids and UDP-glucose transferases in the aphis Sitobion avenae (Homoptera: Aphididae)

UDP-glucose transferases are found in the cytosolic and microsomal fractions of the grain aphid Sitobion avenae F. Gel filtration and SDS-PAGE revealed that the microsomal fraction contained several forms of the enzyme. The molecular weights of the three most active fractions might be 68,000, 66,000...

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Published in:Journal of chemical ecology 1992-07, Vol.18 (7)
Main Authors: Leszczynski, B, Matok, H, Dixon, A.F.G
Format: Article
Language:English
Subjects:
ACIDE ORGANIQUE
ACIDOS ORGANICOS
DESINTOXICACION
DETOXIFICATION
METABOLISME
METABOLISMO
RESISTANCE AUX ORGANISMES NUISIBLES
RESISTENCIA A LAS PLAGAS
SITOBION AVENAE
TRANSFERASAS
TRANSFERASE
TRITICUM AESTIVUM
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Summary:UDP-glucose transferases are found in the cytosolic and microsomal fractions of the grain aphid Sitobion avenae F. Gel filtration and SDS-PAGE revealed that the microsomal fraction contained several forms of the enzyme. The molecular weights of the three most active fractions might be 68,000, 66,000, and 36,500. There was a negative correlation between the enzymes' activity in extracts of aphids and the concentration of DIMBOA-aglucone in the winter wheat variety fed on by the aphid. A strong inhibition of the activity of the UDP-glucose transferases was observed in vitro at a concentration of DIMBOA as low as 0.01 mM. There was a greater activity of the enzymes in aphids fed on seedlings of susceptible than on moderately resistant wheat cultivars. Prolonged feeding on resistant cultivars resulted in a further reduction in the activity of the aphid's enzymes. The significance for cereal aphids of the role of their UDP-glucose transferases in the detoxification of plant allelochemicals and adaptation to resistant varieties of cereals is discussed
ISSN:0098-0331
1573-1561