Genetic diversity of Datisca cannabina-compatible Frankia strains as determined by sequence analysis of the PCR-amplified 16S rRNA gene

• Published in: Applied and Environmental Microbiology 1994-07, Vol.60 (7), p.2371-2376
• Main Authors: Mirza, M.S, Hameed, S, Akkermans, A.D.L
• Format: Article
• Language: English
• Subjects: Actinomycetales - genetics; Actinomycetales - isolation & purification; ADN; Agronomy. Soil science and plant productions; AMPLIFICATION CHAINE POLYMERASE; Animal, plant and microbial ecology; ARN RIBOSOMAL; ARN RIBOSOMIAL; Bacteria; Base Sequence; Biological and medical sciences; Cellular biology; DATISCACEAE; DICOTILEDONEAS; DICOTYLEDONE; DIFERENCIAS BIOLOGICAS; DIFFERENCE BIOLOGIQUE; DNA Primers - genetics; DNA Probes - genetics; DNA, Bacterial - genetics; DNA, Ribosomal - genetics; Economic plant physiology; Flowers & plants; FRANKIA; Fundamental and applied biological sciences. Psychology; GENE; GENES; Genes, Bacterial; Genetic Variation; Laboratorium voor Microbiologie; Microbial ecology; Microbiological Laboratory; Microbiologie; Microbiology; Microflora of plants; Molecular Sequence Data; Nitrogen Fixation; Plants - microbiology; Polymerase Chain Reaction; REACCION DE CADENAS DE POLIMERASA; Ribonucleic acid; RIBOSOMAS; RIBOSOME; RNA; RNA, Bacterial - genetics; RNA, Ribosomal, 16S - genetics; SECUENCIA NUCLEICA; Sequence Homology, Nucleic Acid; SEQUENCE NUCLEIQUE; Soil Microbiology; Soils; Species Specificity; Symbiosis (nodules, symbiotic nitrogen fixation, mycorrhiza...)
• ISSN: 0099-2240; 1098-5336
• DOI: 10.1128/aem.60.7.2371-2376.1994

The presence of Frankia strains in soil samples collected from northern areas of Pakistan was detected by inoculating Coriaria nepalensis and Datisca cannabina plants. The abundance of compatible Frankia strains in some areas was indicated by profuse nodulation of the host plants, whereas soil samples from other localities failed to result in nodulation. An oligonucleotide probe (COR/DAT) directed against the 16S rRNA gene of the endophytes of Coriaria and Datisca spp. that did not cross-react with the RNA gene of Frankia strains isolated from other hosts was developed. Genetic diversity among Frankia strains nodulating D. cannabina was determined by sequence analysis of the partial 16S rRNA gene amplified from nodules induced by soil samples from different localities by PCR. Four types of Frankia sequences and one non-Frankia sequence were detected by hybridization with a Frankia genus probe and the COR/DAT probe as well as by sequence analysis of the cloned PCR products