Oral immunization with recombinant Salmonella typhimurium expressing surface protein antigen A of Steptococcus sobrinus: persistence and induction of humoral responses in rats

Recombinant Salmonella typhimurium has been used as an oral vaccine for various microbial pathogens. Here we report immune responses in Fischer rats orally immunized with a recombinant S. typhimurium strain encoding surface protein antigen A (SpaA) of Streptococcus sobrinus. The attenuated S. typhim...

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Bibliographic Details
Published in:Infection and immunity 1994-08, Vol.62 (8)
Main Authors: Redman, T.K, Harmon, C.C, Michalek, S.M
Format: Article
Language:English
Subjects:
ANTIGENE
ANTIGENOS
BAZO
IMMUNISATION
IMMUNITE HUMORALE
INMUNIDAD HUMORAL
INMUNIZACION
MUTANT
MUTANTES
RAT
RATA
RATE
REPONSE IMMUNITAIRE
RESPUESTA INMUNOLOGICA
SALMONELLA TYPHIMURIUM
SISTEMA LINFATICO
STREPTOCOCCUS
SYSTEME LYMPHATIQUE
VACCIN SYNTHETIQUE
VACUNA SINTETICA
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Summary:Recombinant Salmonella typhimurium has been used as an oral vaccine for various microbial pathogens. Here we report immune responses in Fischer rats orally immunized with a recombinant S. typhimurium strain encoding surface protein antigen A (SpaA) of Streptococcus sobrinus. The attenuated S. typhimurium chi4072 delta cya delta crp delta asd mutant used in this study contains the Asd+ plasmid pYA2905 expressing a fragment of the SpaA protein. Salmonella cells were cleared from spleens by 7 days and from Peyer's patches by 14 days in rats receiving a single oral immunization of 10(9) CFU of chi 4072. In animals receiving multiple i.e., days 0 and 7 or days 0, 7, and 21) immunizations, Salmonella cells were cleared from the Peyer's patches by 25 days following the initial immunization. Antigen-specific systemic and mucosal antibody responses were greater in rats receiving multiple immunizations than in those receiving a single immunization. Serum anti-Salmonella activity was potentiated following boosting on day 21. Mucosal immunoglobulin A antibody responses were also greater in rats receiving multiple immunizations than in rats receiving a single immunization. Anti-Salmonella and anti-Streptococcus immunoglobulin A activity persisted longer in rats boosted on day 21 than in rats immunized on days 0 and 7. These data indicate that oral immunization of rats with tie recombinant S. typhimurium chi 4072 (pYA2905) vaccine induces systemic as well as mucosal antibody responses specific to the Salmonella cells and to the cloned SpaA protein. This is the first report of the use of an attenuated mutant of the murine pathogen S. typhimurium as an oral vaccine in rats
ISSN:0019-9567
1098-5522