Adhesion of germlings of Botrytis cinerea

Adhesion of conidia and germlings of the facultative plant parasite Botrytis cinerea occurs in two distinct stages. The first stage, which occurs immediately upon hydration of conidia and is characterized by relatively weak adhesive forces, appears to involve hydrophobic interactions (R. P. Doss, S....

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Bibliographic Details
Published in:Applied and Environmental Microbiology 1995-01, Vol.61 (1), p.260-265
Main Authors: Doss, R.P. (Horticulture Crops Research Unit, USDA, ARS, Corvallis, OR.), Potter, S.W, Soeldner, A.H, Christian, J.K, Fukunaga, L.E
Format: Article
Language:English
Subjects:
Bacteriology
Biological and medical sciences
Botany
BOTRYTIS CINEREA
Cell Adhesion - drug effects
Cell Adhesion - physiology
Enzymes - pharmacology
Epoxy Compounds - pharmacology
ESPORAS
Flowers & plants
Fundamental and applied biological sciences. Psychology
Fungi
LYCOPERSICON ESCULENTUM
MICELIO
Microbiology
Microscopy, Electron, Scanning
Mitosporic Fungi - physiology
MYCELIUM
Parasites
Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains
Plants - microbiology
POLIMEROS
POLYMERE
PROPIEDADES MECANICAS
PROPRIETE MECANIQUE
SECRECION
SECRETION
SPORE
VERRE
VIDRIO
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Summary:Adhesion of conidia and germlings of the facultative plant parasite Botrytis cinerea occurs in two distinct stages. The first stage, which occurs immediately upon hydration of conidia and is characterized by relatively weak adhesive forces, appears to involve hydrophobic interactions (R. P. Doss, S. W. Potter, G. A. Chastagner, and J. K. Christian, Appl. Environ. Microbiol. 59:1786-1791, 1993). The second stage of adhesion, delayed adhesion, occurs after viable conidia have been incubated for several hours under conditions that promote germination. At this time, the germlings attach strongly to either hydrophobic or hydrophilic substrata. Delayed adhesion involves secretion of an ensheathing film that remains attached to the substratum upon physical removal of the germlings. This fungal sheath, which can be visualized by using interference-contrast light microscopy, scanning electron microscopy, or atomic force microscopy, is 25 to 60 nm thick in the region immediately adjacent to the germ tubes. Germlings are resistant to removal by boiling or by treatment with a number of hydrolytic enzymes, 2.0 M periodic acid, or 1.0 M sulfuric acid. They are readily removed by brief exposure to 1.25 N NaOH. A base-soluble material that adheres to culture flask walls in short-term liquid cultures of B. cinerea is composed of glucose (about 30%), galactosamine (about 3%), and protein (30 to 44%)
ISSN:0099-2240
1098-5336
DOI:10.1128/aem.61.1.260-265.1995