Genetic marking of Lactococcus lactis shows its survival in the human gastrointestinal tract

A human feeding study was performed with Lactococcus lactis TC165.5, which is genetically marked by insertion of the sucrose-nisin conjugative transposon Tn5276 and chromosomal resistance to rifampin and streptomycin. The fate of strain TC165.5 and its nucleic acids was monitored by conventional pla...

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Bibliographic Details
Published in:Applied and Environmental Microbiology 1995-07, Vol.61 (7), p.2771-2774
Main Authors: Klijn, N. (NIZO, Ede, The Netherlands.), Weerkamp, A.H, Vos, W.M. de
Format: Article
Language:English
Subjects:
AMPLIFICATION CHAINE POLYMERASE
APPAREIL DIGESTIF
Bacteria
Bacteriology
Base Sequence
Biological and medical sciences
Digestive system
Digestive System - microbiology
FECES
Fundamental and applied biological sciences. Psychology
GENE
GENERO HUMANO
GENES
GENRE HUMAIN
HECES
Humans
INGESTION DE ALIMENTOS
LACTOCOCCUS LACTIS
Lactococcus lactis - genetics
Lactococcus lactis - physiology
Microbiology
Molecular Sequence Data
NISINA
NYSINE
Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains
Polymerase Chain Reaction
PRISE ALIMENTAIRE (HOMME)
REACCION DE CADENAS DE POLIMERASA
SISTEMA DIGESTIVO
SUPERVIVENCIA
SURVIE
Survival Rate
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Summary:A human feeding study was performed with Lactococcus lactis TC165.5, which is genetically marked by insertion of the sucrose-nisin conjugative transposon Tn5276 and chromosomal resistance to rifampin and streptomycin. The fate of strain TC165.5 and its nucleic acids was monitored by conventional plating methods and by molecular detection techniques based on specific PCR amplification of the nisin (nisA) gene from DNA extracted from human feces. A method was developed for the efficient extraction of microbial DNA from human feces. The results show that a fraction of viable cells of L. lactis TC165.5 survived passage through the human gastrointestinal tract. Only cells that passed within 3 days of ingestion could be recovered from the feces of the volunteers, and they accounted for approximately 1% of the total number of cells consumed. The presence of nisA in DNA extracted from feces could be detected up to 4 days, when viable cells were no longer present
ISSN:0099-2240
1098-5336
DOI:10.1128/aem.61.7.2771-2774.1995