Rhizobium tropici chromosomal citrate synthase gene

Two genes encoding citrate synthase, a key enzyme in the Krebs cycle, have been found in Rhizobium tropici. One of them is in the bacterial chromosome, while the other is in the symbiotic plasmid. We sequenced the chromosomal gene and found that it is very similar to the previously reported plasmidi...

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Bibliographic Details
Published in:Applied and Environmental Microbiology 1995-11, Vol.61 (11), p.3992-3997
Main Authors: Hernandez-Lucas, I. (Universidad Nacional Autonoma de Mexico, Cuernavaca, Morelos, Mexico.), Pardo, M.A, Segovia, L, Miranda, J, Martinez-Romero, E
Format: Article
Language:English
Subjects:
ACTIVIDAD ENZIMATICA
ACTIVITE ENZYMATIQUE
Amino Acid Sequence
Bacteria
Bacteriology
Base Sequence
Biological and medical sciences
CHROMOSOME
Chromosomes, Bacterial - genetics
Citrate (si)-Synthase - genetics
COMPOSICION QUIMICA
COMPOSITION CHIMIQUE
CROMOSOMAS
DNA Primers - genetics
DNA, Bacterial - genetics
Enzymes
Escherichia coli - enzymology
Escherichia coli - genetics
FIJACION DEL NITROGENO
FIXATION DE L'AZOTE
FLORA MICROBIANA
FLORE MICROBIENNE
FORMATION DE NODOSITES
Fundamental and applied biological sciences. Psychology
GENE
GENES
Genes, Bacterial
Genetic Complementation Test
Genetics
LIASAS
LYASE
Microbiology
Molecular biology
Molecular Sequence Data
Mutagenesis, Site-Directed
MUTANT
MUTANTES
NODULACION
PHASEOLUS VULGARIS
Phenotype
Plasmids - genetics
RHIZOBIUM
Rhizobium - enzymology
Rhizobium - genetics
Rhizobium tropici
SECUENCIA NUCLEICA
Sequence Homology, Amino Acid
Sequence Homology, Nucleic Acid
SEQUENCE NUCLEIQUE
Symbiosis
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Summary:Two genes encoding citrate synthase, a key enzyme in the Krebs cycle, have been found in Rhizobium tropici. One of them is in the bacterial chromosome, while the other is in the symbiotic plasmid. We sequenced the chromosomal gene and found that it is very similar to the previously reported plasmidic gene sequence in its structural region but not in its regulatory region. The chromosomal gene is able to complement an Escherichia coli citrate synthase mutant. In R. tropici, a mutant in the chromosomal citrate synthase gene has a diminished citrate synthase activity (in free-living bacteria), a diminished nodulation capacity, and forms nitrogen-fixing nodules. In contrast, the citrate synthase double mutant forms ineffective nodules devoid of bacteroids and forms less nodules than the single chromosomal mutant. It is inferred that both genes are functional and required during the nodulation process in R. tropici
ISSN:0099-2240
1098-5336
DOI:10.1128/aem.61.11.3992-3997.1995