Salmonella strain secreting active listeriolysin changes its intracellular localization

We describe the construction of an attenuated Salmonella dublin aroA strain which secretes via the Escherichia coli hemolysin secretion machinery an active hybrid cytolysin consisting of listeriolysin from Listeria monocytogenes and the C-terminal secretion signal of E. coli hemolysin. This hemolyti...

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Bibliographic Details
Published in:Infection and Immunity 1995-10, Vol.63 (10), p.4202-4205
Main Authors: Gentschev, I. (Universitat Ulm, Ulm, Germany.), Sokolovic, Z, Mollenkopf, H.J, Hess, J, Kaufmann, S.H.E, Kuhn, M, Krohne, G.F, Goebel, W
Format: Article
Language:English
Subjects:
Animals
Bacterial Toxins
Bacteriology
Base Sequence
Biological and medical sciences
Cell Line
CITOPLASMA
CLONACION
CLONAGE
CYTOPLASME
ESCHERICHIA COLI
Fundamental and applied biological sciences. Psychology
Heat-Shock Proteins - physiology
Hemolysin Proteins - physiology
LISTERIA MONOCYTOGENES
MACROFAGOS
MACROPHAGE
Macrophages - microbiology
Mice
Microbiology
Molecular Sequence Data
Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains
PROTEINAS
PROTEINE
Salmonella - physiology
SALMONELLA DUBLIN
SECRECION
SECRETION
TOXINAS
TOXINE
VACUOLA
VACUOLE
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Summary:We describe the construction of an attenuated Salmonella dublin aroA strain which secretes via the Escherichia coli hemolysin secretion machinery an active hybrid cytolysin consisting of listeriolysin from Listeria monocytogenes and the C-terminal secretion signal of E. coli hemolysin. This hemolytic S. dublin strain is partially released into the cytoplasm of the host cell following uptake by J774 macrophage cells, whereas the nonhemolytic control S. dublin aroA strain remains in the phagosome
ISSN:0019-9567
1098-5522
DOI:10.1128/iai.63.10.4202-4205.1995