Ribotype diversity of Listeria monocytogenes strains associated with outbreaks of listeriosis in ruminants

Ribotyping is a molecular method for the characterization, identification, and typing of bacterial isolates that has value in epidemiological studies. To demonstrate the utility of this technique for typing of Listeria monocytogenes, four outbreaks of epizootic listeriosis in ruminants were investig...

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Bibliographic Details
Published in:Journal of Clinical Microbiology 1996-05, Vol.34 (5), p.1086-1090
Main Authors: Wiedmann, M. (Cornell University, Ithaca, NY.), Bruce, J.L, Knorr, R, Bodis, M, Cole, E.M, McDowell, C.I, McDonough, P.L, Batt, C.A
Format: Article
Language:English
Subjects:
Abortion, Veterinary - epidemiology
Abortion, Veterinary - microbiology
Animal bacterial diseases
Animal Feed - microbiology
Animals
Bacterial diseases
Bacterial Typing Techniques
Biological and medical sciences
Cattle
Cattle Diseases - epidemiology
Cattle Diseases - microbiology
Disease Outbreaks - veterinary
Female
Genetic Variation
Goat Diseases - epidemiology
Goat Diseases - microbiology
Goats
Infectious diseases
LISTERIA MONOCYTOGENES
Listeria monocytogenes - classification
Listeria monocytogenes - genetics
Listeria monocytogenes - isolation & purification
LISTERIOSE
LISTERIOSIS
Listeriosis - epidemiology
Listeriosis - microbiology
Listeriosis - veterinary
Medical sciences
Polymerase Chain Reaction
Pregnancy
RUMIANTE
RUMINANT
Ruminantia
Sheep
Sheep Diseases - epidemiology
Sheep Diseases - microbiology
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Description
Summary:Ribotyping is a molecular method for the characterization, identification, and typing of bacterial isolates that has value in epidemiological studies. To demonstrate the utility of this technique for typing of Listeria monocytogenes, four outbreaks of epizootic listeriosis in ruminants were investigated through coordinated detection and characterization methods utilizing classical microbiology and nucleic acid-based techniques. L. monocytogenes strains isolated from clinical samples and the silage consumed by the affected animals were ribotyped to establish the causal relationship between feed and the disease outbreak. For all but one outbreak, we were able to isolate L. monocytogenes strains represented by the same ribotype from both clinical and silage samples. Additional L. monocytogenes strains with ribotypes different from those of the respective clinical samples were isolated from all silage samples. This indicates that a diverse population of L. monocytogenes strains exists in farm environments, of which some may be more likely than others to cause disease
ISSN:0095-1137
1098-660X
DOI:10.1128/JCM.34.5.1086-1090.1996