Vitamin B

To investigate the effect of B and/or folic acid supplementation on genome-wide DNA methylation. We performed Infinium HumanMethylation450 BeadChip (Zymo Research, CA, USA) assay in children supplemented with B and/or folic acid (n = 12 in each group) and investigated the functional mechanism of sel...

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Bibliographic Details
Published in:Epigenomics 2018-01, Vol.10 (1), p.71-90
Main Authors: Yadav, Dilip K, Shrestha, Smeeta, Lillycrop, Karen A, Joglekar, Charu V, Pan, Hong, Holbrook, Joanna D, Fall, Caroline HD, Yajnik, Chittaranjan S, Chandak, Giriraj R
Format: Article
Language:English
Subjects:
DNA methylation
folic acid
miRNAs
molecular mechanisms
supplementation
Type 2 diabetes
vitamin B
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Summary:To investigate the effect of B and/or folic acid supplementation on genome-wide DNA methylation. We performed Infinium HumanMethylation450 BeadChip (Zymo Research, CA, USA) assay in children supplemented with B and/or folic acid (n = 12 in each group) and investigated the functional mechanism of selected differentially methylated loci. We noted significant methylation changes postsupplementation in B (589 differentially methylated CpGs and 2892 regions) and B + folic acid (169 differentially methylated CpGs and 3241 regions) groups. Type 2 diabetes-associated genes and and a miRNA, were further investigated in another B -supplementation cohort. We also demonstrate that methylation influences expression and , , and are direct targets of . B supplementation influences regulation of several metabolically important Type 2 diabetes-associated genes through methylation of . Hence, our study provides novel epigenetic explanation for the association between disordered one carbon metabolism and risk of adiposity, insulin resistance and diabetes and has translational potential.
ISSN:1750-1911
1750-192X
DOI:10.2217/epi-2017-0102