DNA methylation and gene expression profiling reveal

To integrate transcriptomic and DNA-methylomic measurements on varicose versus normal veins using a systems biological analysis to shed light on the interplay between genetic and epigenetic factors. Differential expression and methylation were measured using microarrays, supported by real-time quant...

Full description

Saved in:
Bibliographic Details
Published in:Epigenomics 2018-08
Main Authors: Smetanina, Mariya A, Kel, Alexander E, Sevost'ianova, Ksenia S, Maiborodin, Igor V, Shevela, Andrey I, Zolotukhin, Igor A, Stegmaier, Philip, Filipenko, Maxim L
Format: Article
Language:English
Subjects:
DNA methylation
extracellular matrix
gene expression
systems biological analysis
varicose vein(s)
vascular remodeling
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:To integrate transcriptomic and DNA-methylomic measurements on varicose versus normal veins using a systems biological analysis to shed light on the interplay between genetic and epigenetic factors. Differential expression and methylation were measured using microarrays, supported by real-time quantitative PCR and immunohistochemistry confirmation for relevant gene products. A systems biological 'upstream analysis' was further applied. We identified several potential key players contributing to extracellular matrix remodeling in varicose veins. Specifically, our analysis suggests acting as a master regulator, upstream of integrins, of the cellular network affecting the varicose vein condition. Possible mechanism and pathogenic model were outlined. A coherent model proposed incorporates the relevant signaling networks and will hopefully aid further studies on varicose vein pathogenesis.
ISSN:1750-1911
1750-192X
DOI:10.2217/epi-2018-0001