Multiple-locus variable number tandem repeat analysis is superior to

Assess the best approach to type methicillin-resistant (MRSA), ( ) typing, multiple-locus variable number tandem repeat analysis (MLVA) or both. Discriminatory power of typing and MLVA was determined using 20,771 MRSA isolates. There were twice as many MLVA types (MTs) as types present in the collec...

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Bibliographic Details
Published in:Future microbiology 2015-07, Vol.10 (7), p.1155-1162
Main Authors: Bosch, Thijs, Pluister, Gerlinde N, van Luit, Martijn, Landman, Fabian, van Santen-Verheuvel, Marga, Schot, Corrie, Witteveen, Sandra, van der Zwaluw, Kim, Heck, Max EOC, Schouls, Leo M
Format: Article
Language:English
Subjects:
diversity
MLVA
MRSA
surveillance
typing
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Summary:Assess the best approach to type methicillin-resistant (MRSA), ( ) typing, multiple-locus variable number tandem repeat analysis (MLVA) or both. Discriminatory power of typing and MLVA was determined using 20,771 MRSA isolates. There were twice as many MLVA types (MTs) as types present in the collection. Among the top 70% of the isolates, 37 types and 139 MTs were found. MLVA diversity among the top-10 types was high (diversity index 0.96), while diversity among the top-10 MTs was much lower (diversity index 0.83). The probability that two MRSA isolates with the same type also had the same MT was low (Wallace's coefficient 0.27). By contrast, most MRSA isolates yielding the same MT also had the same type (Wallace's coefficient 0.90). MLVA is superior to typing and will suffice to characterize MRSA isolates for surveillance.
ISSN:1746-0913
1746-0921
DOI:10.2217/fmb.15.35