Selenium reduces enterohemorrhagic

This study investigated the efficacy of selenium (Se) in reducing O157:H7 verotoxin production and toxin gene expression. Additionally, the effect of Se on globotriaosylceramide (Gb3) receptor in human lymphoma cells was determined. The effect of Se on verotoxin synthesis was determined by standard...

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Bibliographic Details
Published in:Future microbiology 2016-06, Vol.11 (6), p.745-756
Main Authors: Surendran-Nair, Meera, Kollanoor-Johny, Anup, Ananda-Baskaran, Sangeetha, Norris, Carol, Lee, Ji-Young, Venkitanarayanan, Kumar
Format: Article
Language:English
Subjects:
Gb3 receptor
O157:H7
selenium
verotoxin
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Summary:This study investigated the efficacy of selenium (Se) in reducing O157:H7 verotoxin production and toxin gene expression. Additionally, the effect of Se on globotriaosylceramide (Gb3) receptor in human lymphoma cells was determined. The effect of Se on verotoxin synthesis was determined by standard ELISA, whereas its effect on Gb3 receptor was determined by flow cytometry and real-time quantitative PCR. Se reduced extracellular and intracellular verotoxin concentration by 40-60% and 80-90%, respectively (p < 0.05), and downregulated verotoxin genes (p < 0.05). Se reduced Gb3 receptor synthesis in lymphoma cells, and real-time quantitative PCR data revealed a significant downregulation of synthase gene ( ) involved in Gb3 synthesis. Further studies are warranted to validate these results in an appropriate animal model.
ISSN:1746-0913
1746-0921
DOI:10.2217/fmb.16.16