Alterations of zinc homeostasis in response to

To evaluate alterations of zinc homeostasis in macrophages exposed to Using a fluorescent zinc probe-based flow cytometry and atomic absorption spectrometry, zinc levels were evaluated in J774.A1 cell lines exposed to H99 cells. The transcription profile of macrophage zinc related homeostasis genes...

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Bibliographic Details
Published in:Future microbiology 2017-05, Vol.12 (6), p.491-504
Main Authors: Dos Santos, Francine Melise, Piffer, Alícia Corbellini, Schneider, Rafael de Oliveira, Ribeiro, Nicole Sartori, Garcia, Ane Wichine Acosta, Schrank, Augusto, Kmetzsch, Lívia, Vainstein, Marilene Henning, Staats, Charley Christian
Format: Article
Language:English
Subjects:
nutritional immunity
zinc
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Summary:To evaluate alterations of zinc homeostasis in macrophages exposed to Using a fluorescent zinc probe-based flow cytometry and atomic absorption spectrometry, zinc levels were evaluated in J774.A1 cell lines exposed to H99 cells. The transcription profile of macrophage zinc related homeostasis genes - metallothioneins and zinc transporters (ZnTs) of the SLC30 and SLC39 (Zrt-Irt-protein) families - was analyzed by quantitative PCR. : Macrophage intracellular labile zinc levels decreased following exposure to . A significant decrease in transcription levels was detected in specific ZnTs from both the Zrt-Irt-protein and ZnT families, especially 24 h after infection. These findings suggest that macrophages may exhibit zinc depletion in response to infection.
ISSN:1746-0913
1746-0921
DOI:10.2217/fmb-2016-0160