Modulatory effects of verapamil in rifampicin activity against

To evaluate modulatory effect of verapamil (VP) in rifampicin (RIF) activity and its effect in efflux pumps (EPs) transcript levels in . RIF and VP minimal inhibitory concentration, combinatory effect and detection of mutations were determined in 16 isolates. EPs transcript levels were determined in...

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Bibliographic Details
Published in:Future microbiology 2019-02, Vol.14 (3), p.185-194
Main Authors: Caleffi-Ferracioli, Katiany R, Cardoso, Rosilene F, de Souza, João VP, Murase, Letícia S, Canezin, Pedro H, Scodro, Regiane BL, LD Siqueira, Vera, Pavan, Fernando R
Format: Article
Language:English
Subjects:
efflux pump inhibitor
gene regulation
multidrug transporters
real-time PCR
resistance
rifampicin
synergism
tuberculosis
verapamil
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Summary:To evaluate modulatory effect of verapamil (VP) in rifampicin (RIF) activity and its effect in efflux pumps (EPs) transcript levels in . RIF and VP minimal inhibitory concentration, combinatory effect and detection of mutations were determined in 16 isolates. EPs transcript levels were determined in four isolates by real-time PCR after exposure to drugs. VP showed good combinatory effect among RIF-resistant isolates. This effect was also observed in the relative transcript levels of EPs, mainly after 72 h of exposure, depending on the EP gene, genotype and the resistance profile of the isolate. Additional regulatory mechanisms in the EP activities, as well as, interactions with other drug-specific resistance mechanisms need further investigation in
ISSN:1746-0913
1746-0921
DOI:10.2217/fmb-2018-0277