Copper oxide nanoparticles recruit macrophages and modulate nitric oxide, proinflammatory cytokines and PGE

: In the present study, we examine the effects of copper oxide nanoparticles (CuNP) on macrophage immune response and the signaling pathways involved. A peritonitis model was used to determine immune cells recruitment, while primary macrophages were used as an model for the cellular and molecular an...

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Bibliographic Details
Published in:Nanomedicine (London, England) England), 2016-05, Vol.11 (10), p.1237-1251
Main Authors: Arancibia, Sergio, Barrientos, Andrea, Torrejón, Javiera, Escobar, Alejandro, Beltrán, Caroll J
Format: Article
Language:English
Subjects:
cyclooxygenase 2
immunotoxicology
inducible nitric oxide synthase
nanomedicine
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Summary:: In the present study, we examine the effects of copper oxide nanoparticles (CuNP) on macrophage immune response and the signaling pathways involved. A peritonitis model was used to determine immune cells recruitment, while primary macrophages were used as an model for the cellular and molecular analysis. , CuNP induce significant macrophages recruitment to the site of injection. , in LPS-stimulated primary macrophages, the co-treatment with CuNP inhibited the production of NO in a dose-dependent manner. The mechanism underlying NO and proinflammatory cytokines inhibition was associated with an increased arginase activity. Macrophage stimulation with CuNP did not provoke any cytokine secretion; however, arginase inhibition promoted TNFα and MIP-1β production. In addition, CuNP induced the expression of COX-2 and the production of PGE through arginase activation. Our results demonstrate that CuNP activate arginase and suppress macrophage innate immune response.
ISSN:1743-5889
1748-6963
DOI:10.2217/nnm.16.39