ζ potential changing nanoparticles as cystic fibrosis transmembrane conductance regulator gene delivery system: an

Aim of the study was the development of ζ potential changing nanoparticles as gene delivery system for the cystic fibrosis transmembrane conductance regulator gene. Chitosan and carboxymethyl cellulose were modified with phosphotyrosine, a substrate for the brush border enzyme alkaline phosphatase....

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Bibliographic Details
Published in:Nanomedicine (London, England) England), 2017-11, Vol.12 (22), p.2713-2724
Main Authors: Prüfert, Felix, Bonengel, Sonja, Köllner, Saskia, Griesser, Janine, Wilcox, Matthew D, Chater, Peter I, Pearson, Jeffrey P, Bernkop-Schnürch, Andreas
Format: Article
Language:English
Subjects:
cystic fibrosis transmembrane conductance regulator
gene delivery
phosphatase
phosphotyrosine
ζ potential changing nanoparticles
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Summary:Aim of the study was the development of ζ potential changing nanoparticles as gene delivery system for the cystic fibrosis transmembrane conductance regulator gene. Chitosan and carboxymethyl cellulose were modified with phosphotyrosine, a substrate for the brush border enzyme alkaline phosphatase. With these synthesized derivatives, different nanoparticle formulations, including the cystic fibrosis transmembrane conductance regulator gene were prepared by ionic gelation. A change from negative to positive ζ potential after enzymatic cleavage could be observed. Transfection studies with HEK-293 and Caco-2 cells showed transfection rates comparable to Lipofectamine 2000. Transfection efficiencies were significantly decreased when phosphate cleavage and thus ζ potential change was inhibited by phosphatase inhibitor. The developed nanoparticles represent a promising gene delivery system.
ISSN:1743-5889
1748-6963
DOI:10.2217/nnm-2017-0115