Detection of p190BCR-ABL and p210BCR-ABL fusion transcripts in patients with chronic myeloid leukemia (CML) using qualitative RT-PCR/Frecuencia de los transcriptos p190BCR-ABL y p210BCR-ABL en una poblacion colombiana con leucemia mieloide cronica (LMC) usando RT-PCR cualitativa/Frequencia dos transcritos p190BCR-ABL e p210BCR-ABL numa populacao colombiana com leucemia mieloide cronica (LMC) usando RT-PCR qualitativa

Introduction: Chronic myelogenous leukemia (CML) is characterized by the presence of the Philadelphia chromosome (Ph), resulting from the balanced reciprocal translocation t(9;22)(q34;ql 1). This marker chromosome is found less frequently in patients suffering from acute lymphoblastic leukemia. Obje...

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Published in:Iatreia (Medellín, Colombia) Colombia), 2014-10, Vol.27 (4), p.398
Main Authors: Gaviria, Gloria Ramirez, Garces, Jorge Humberto Botero, A., Carmen Gloria Artigas, Sierra, Jorge Cuervo, Sanchez, Margarita Sierra, Muskus, Carlos Enrique, Cuellar-Ambrosi, Francisco, Palacio, Gonzalo Vasquez, Bonilla, Carlos Alberto Aya, Muneton, Carlos Mario, Torres, Jose Domingo
Format: Article
Language:Spanish
Subjects:
Analysis
Care and treatment
Chronic myeloid leukemia
Health aspects
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Summary:Introduction: Chronic myelogenous leukemia (CML) is characterized by the presence of the Philadelphia chromosome (Ph), resulting from the balanced reciprocal translocation t(9;22)(q34;ql 1). This marker chromosome is found less frequently in patients suffering from acute lymphoblastic leukemia. Objective: To determine the frequency of BCR-ABL gene fusions encoding the [p210.sup.BCR-ABL] y [p190.sup.BCR-ABL] transcripts in Colombian patients diagnosed with CML in different stages of the disease and/or its treatment. Materials and methods: Cross sectional, descriptive study of thirty one CML patients (aged 15-78). Analysis was carried out through qualitative nested PCR for the isoforms P210 BCR-ABL (b3a2 e b2a2) and Pi90 BCR-ABL (eia2). and based on peripheral blood samples. Results: In 29 of the 31 patients (93.6%) transcript p21 oBCR'ABL was detected: b2a2 and b3a2 gene fusions and the coexpression b3a2 y b2a2 were identified in 55.2% (16/29), 34.5% (10/29) and 10.3% (3/29) of the cases, respectively. Conclusion: b2a2 gene fusion was the most frequent in this CML population. KEYWORDS Leukemia, Myelogenous, Chronic; Nested PCR: Fusion Proteins, bcr-abl Introduccion: la leucemia mieloide cronica (LMC) se caracteriza por la presencia del cromosoma Filadelfia (Ph) que resulta de la translocacion reciproca balanceada t(9;22)(q34;ql 1); este marcador cromosomico se encuentra con menor frecuencia en pacientes con leucemia linfoide aguda (LLA). Objetivo: determinar la frecuencia de las fusiones genicas BCR-ABL, que codifican para los transcriptos [p210.sup.BCR-ABL] y [p190.sup.BCR-ABL] en pacientes colombianos con diagnostico de LMC. en diferentes fases de la enfermedad o de su tratamiento. Materiales y metodos: estudio descriptivo de corte transversal de 31 pacientes con LMC (15-78 anos). El analisis se hizo a partir de muestras de sangre periferica con la tecnica PCR anidada cualitativa para las isoformas P210 BCR-ABL (b3a2 e b2a2) y P190 BCR-ABL (e 1 a2). Resultados: se detecto el transcripto [p210.sup.BCR-ABL] en 29 de los 31 casos (93.6%). En ellos se identificaron las fusiones genicas b2a2 (16/29; 55,2%), b3a2 (10/29; 34,5%) y la coexpresion b3a2 y b2a2 (3/29; 10,3%). Conclusion: la fusion genica b2a2 fue la mas frecuente en esta poblacion con LMC. PALABRAS CLAVE Leucemia Mieloide Cronica; PCR Anidada; Proteinas de Fusion bcr-abl Introducao: a leucemia mieloide cronica (LMC) caracteriza-se pela presenca do cromossomo Filadelfia (Ph) que resulta da translocac
ISSN:0121-0793