Expression levels of complement regulatory proteins

Background: Altered regulation of the complement system is associated with multiple kidney diseases. CD35, CD55 and CD59 regulate the complement system, and changes in their expression have previously been linked with kidney disease. This study assessed whether changes in the expression levels of th...

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Published in:International journal of general medicine 2019-09, p.343
Main Authors: Eid, Hanaa Mohammed, Alhabibi, Alshaymaa M, Ibrahim, Amal H, Haliem, Naglaa F. Abd El, Gouda, Rasha Mahmoud, Eldewi, Dalia Mahmoud, Sayed, El, Ahmed, Sammar, Mahmoud, Kasim, Hassan, Mohammed Abd El Malik, Sadek, Sanaa Mohammed El
Format: Article
Language:English
Subjects:
Child health
Chronic kidney failure
EDTA
Fluorescence
Kidney diseases
Lymphocytes
Medical research
Pediatrics
Proteins
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Summary:Background: Altered regulation of the complement system is associated with multiple kidney diseases. CD35, CD55 and CD59 regulate the complement system, and changes in their expression have previously been linked with kidney disease. This study assessed whether changes in the expression levels of these proteins are associated specifically with chronic kidney disease (CKD) to understand its pathogenesis. Materials and methods: Sixty CKD patients and 60 age-matched controls were enrolled and divided into two groups: Group I (n=30 pediatric patients and n=30 controls) and Group II (n=30 adult patients and n=30 controls). The expression of CD35, CD55 and CD59 on peripheral blood cells was evaluated by flow cytometry as the proportion of positive cells expressing the marker and mean fluorescence intensity (MFI), also the relation of these markers to the stage of CKD was also evaluated. Results: Pediatric and adult CKD patients had significantly lower proportion of erythrocytes expressing CD35,CD55 and CD59 than healthy controls (P
ISSN:1178-7074
1178-7074