A proline derivative-enriched methanol fraction from Sideroxylon obtusifolium leaves

It was previously demonstrated that the methanol fraction of Sideroxylon obtusifolium (MFSOL) promoted anti-inflammatory and healing activity in excisional wounds. Thus, the present work investigated the healing effects of MFSOL on human keratinocyte cells (HaCaT) and experimental burn model injurie...

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Bibliographic Details
Published in:Brazilian journal of medical and biological research 2021-09, Vol.54 (9)
Main Authors: Souza, T.F.G, Macedo, F.S, Silva, L.M.A, Alencar, N.M.N, Rangel, G.F.P, Alves, A.P.N.N, Silveira, E.R, Montenegro, R.C, Viana, G.S.B, Pierdona, T.M, Aquino, P.E.A, Duarte, R.S, Wilke, D.V
Format: Article
Language:English
Subjects:
Analysis
Burns and scalds
Instrument industry
International economic relations
Methanol
Proline
Silver sulfadiazine
Sulfadiazine
Vascular endothelial growth factor
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Summary:It was previously demonstrated that the methanol fraction of Sideroxylon obtusifolium (MFSOL) promoted anti-inflammatory and healing activity in excisional wounds. Thus, the present work investigated the healing effects of MFSOL on human keratinocyte cells (HaCaT) and experimental burn model injuries. HaCaT cells were used to study MFSOL's effect on cell migration and proliferation rates. Female Swiss mice were subjected to a second-degree superficial burn protocol and divided into four treatment groups: Vehicle, 1.0% silver sulfadiazine, and 0.5 or 1.0% MFSOL Cream (CrMFSOL). Samples were collected to quantify the inflammatory mediators, and histological analyses were performed after 3, 7, and 14 days. The results showed that MFSOL (50 [micro]g/mL) stimulated HaCaT cells by increasing proliferation and migration rates. Moreover, 0.5% CrMFSOL attenuated myeloperoxidase (MPO) activity and also stimulated the release of interleukin (IL)-1[beta] and IL-10 after 3 days of treatment. CrMFSOL (0.5%) also enhanced wound contraction, promoted improvement of tissue remodeling, and increased collagen production after 7 days and VEGF release after 14 days. Therefore, MFSOL stimulated human keratinocyte (HaCaT) cells and improved wound healing via modulation of inflammatory mediators of burn injuries. Keywords: Sideroxylon obtusifolium (Roem. & Schult.); Methanol fraction; Human keratinocytes; Wound healing; Burn wounds
ISSN:0100-879X