Use PCR & a Single Hair to Produce a "DNA Fingerprint"

Presents a laboratory procedure that involves students extracting their own DNA from a single hair follicle, using the polymerase chain reaction (PCR) to amplify a polymorphic locus, performing electrophoresis on the PCR products on an agarose gel, and visualizing the alleles to generate a "DNA...

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Bibliographic Details
Published in:The American biology teacher 1997-03, Vol.59 (3), p.172-178
Main Authors: Campbell, A. Malcolm, John H. Williamson, Diane Padula, Steve Sundby
Format: Article
Language:English
Subjects:
Alleles
Biology
Biotechnology
Bromides
DNA
DNA identification
DNA testing
Follicles
Gels
Genetic loci
Genetics
Hair
Hair follicles
Higher Education
How-To-Do-It
Laboratory Equipment
Polymerase chain reaction
Science Experiments
Science Instruction
Study and teaching
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Summary:Presents a laboratory procedure that involves students extracting their own DNA from a single hair follicle, using the polymerase chain reaction (PCR) to amplify a polymorphic locus, performing electrophoresis on the PCR products on an agarose gel, and visualizing the alleles to generate a "DNA fingerprint." Discusses theoretical background, teaching applications, timetable and equipment, beginner's protocol, and advanced protocol. (JRH)
ISSN:0002-7685
1938-4211
DOI:10.2307/4450275