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We wanted to investigate effects of vitamin D.sub.3 (25(OH)D.sub.3 and 1.25(OH).sub.2 D.sub.3) on inflammatory cytokine expression in both activated and non-activated M[phi]. Mononuclear cells, isolated from healthy donor buffy coats were cultured for a 6-day differentiation-period. Fully differenti...

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Bibliographic Details
Published in:PloS one 2019-04, Vol.14 (4), p.e0215383
Main Authors: Rafique, Aisha, Rejnmark, Lars, Heickendorff, Lene, Møller, Holger Jon
Format: Article
Language:English
Subjects:
Biochemistry
Care and treatment
Cytochrome P-450
Cytokines
Development and progression
EDTA
Enzyme-linked immunosorbent assay
Gene expression
Genes
Health aspects
Immune system
Inflammation
Macrophages
Physiological aspects
Polymerase chain reaction
Tumor necrosis factor
Vitamin D3
Vitamins
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Summary:We wanted to investigate effects of vitamin D.sub.3 (25(OH)D.sub.3 and 1.25(OH).sub.2 D.sub.3) on inflammatory cytokine expression in both activated and non-activated M[phi]. Mononuclear cells, isolated from healthy donor buffy coats were cultured for a 6-day differentiation-period. Fully differentiated M[phi] were pre-treated with either 25(OH)D.sub.3 or 1.25(OH).sub.2 D.sub.3 for (4, 12 or 24 hours) +/-LPS challenge for 4 hours.sub.. Gene expression analyses of VDR, Cyp27b1 and pro-inflammatory markers TNF-[alpha], IL-6, NF-[kappa]B, MCP-1, was performed using RT-quantitative PCR. TNF-[alpha] protein levels from M[phi] culture media were analysed by ELISA. Both 25(OH)D.sub.3 and 1.25(OH).sub.2 D.sub.3 significantly inhibited TNF-[alpha] expression in both LPS-stimulated and unstimulated M[phi]. Also, NF-[kappa]B, and to a lesser extend IL-6 and MCP-1 were inhibited. LPS up-regulated Cyp27b1 gene expression which was partly reverted by 1.25(OH).sub.2 D.sub.3. These data show anti-inflammatory effects of vitamin D.sub.3 (25(OH)D.sub.3 and 1.25(OH).sub.2 D.sub.3) in human macrophages, and support, that means for targeting high dose vitamin D.sub.3 to the immune system may have beneficial clinical effect in inflammatory conditions.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0215383