Biological effect and molecular docking of anticancer palladium and platinum complexes with morpholine dithiocarbamate on human serum albumin as a blood carrier protein

• Published in: Canadian journal of physiology and pharmacology 2018-12, Vol.96 (12), p.1276-1285
• Main Authors: Hosseini, Seyed Ali, Eslami Moghadam, Mahboube, Saeidifar, Maryam, Saboury, Ali Akbar
• Format: Article
• Language: English
• Subjects: Absorption; activité antifongique et antibactérienne; Albumin; Antibacterial activity; Antibacterial agents; Antifungal agents; antifungal and antibacterial activity; Antineoplastic Agents - pharmacology; arrimage moléculaire; Ashes; Blood Proteins - metabolism; Cancer; Cancer treatment; Carrier Proteins - metabolism; Cell Line, Tumor; Chronic myeloid leukemia; Circular Dichroism; Cisplatin; Cisplatin - metabolism; Cisplatin - pharmacology; complexes Pt et Pd; Cytotoxicity; Dichroism; dithiocarbamate; Drug Interactions - physiology; Emission analysis; Fluorescence; fluorescence and circular dichroism spectroscopy; Fungicides; Health aspects; HSA binding; Human serum albumin; Humans; Innovations; K562 Cells; Leukemia; Leukemia, Myelogenous, Chronic, BCR-ABL Positive - drug therapy; Leukemia, Myelogenous, Chronic, BCR-ABL Positive - metabolism; liaison à l’albumine sérique humaine; Microorganisms; molecular docking; Molecular Docking Simulation; Morpholines - pharmacology; Myeloid leukemia; Organoplatinum Compounds - pharmacology; Palladium; Palladium - pharmacology; Pharmacology; Physiology; Platinum; Platinum compounds; Protein Binding - physiology; Pt and Pd complexes; Serum albumin; Serum Albumin, Human - metabolism; spectroscopie par fluorescence et dichroïsme circulaire; Spectroscopy; Thermal stability
• ISSN: 0008-4212; 1205-7541
• DOI: 10.1139/cjpp-2017-0693

The aim of this study was to examine the interaction of [Pd(2,2′-bipyridine) (morpholinedithiocarbamate)]NO 3 and [Pt (2,2′-bipyridine)(morpholinedithiocarbamate)]NO 3 with human serum albumin under physiological conditions by using fluorescence, absorption, and circular dichroism spectroscopic techniques. Spectroscopic analysis of the emission quenching at different temperatures demonstrated that the quenching mechanism was static quenching. From the circular dichroism results, thermal stability study, it was found that the interaction of the complexes with human serum albumin caused a conformational change of the protein reversibly. These 2 anticancer Pd and Pt complexes were activated against chronic myelogenous leukemia cell line K562, so that 50% cytotoxic concentration values of 16 and 26 μM for Pd and Pt complexes, respectively, were observed, which were much lower than that of cisplatin (154 μM). Biological activities of both Pd and Pt complexes were also assayed against selective microorganisms by the disc diffusion method. These results showed that the Pd(II) complex is antifungal agent but Pt(II) complex has antibacterial activity. Also, the interaction of both metal derivative complexes was studied by molecular docking. Complementary molecular docking results may be useful to determine the binding mechanism of human serum albumin in pharmaceutical and biophysical studies providing new insight in the novel pharmacology.