A Comprehensive DNA Barcode Library for the Looper Moths

The construction of comprehensive reference libraries is essential to foster the development of DNA barcoding as a tool for monitoring biodiversity and detecting invasive species. The looper moths of British Columbia (BC), Canada present a challenging case for species discrimination via DNA barcodin...

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Bibliographic Details
Published in:PloS one 2011-03, Vol.6 (3), p.e18290
Main Authors: deWaard, Jeremy R, Hebert, Paul D. N, Humble, Leland M
Format: Article
Language:English
Subjects:
Analysis
Bar codes
DNA
Geometrid moths
Online Access:Get full text
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Summary:The construction of comprehensive reference libraries is essential to foster the development of DNA barcoding as a tool for monitoring biodiversity and detecting invasive species. The looper moths of British Columbia (BC), Canada present a challenging case for species discrimination via DNA barcoding due to their considerable diversity and limited taxonomic maturity. By analyzing specimens held in national and regional natural history collections, we assemble barcode records from representatives of 400 species from BC and surrounding provinces, territories and states. Sequence variation in the barcode region unambiguously discriminates over 93% of these 400 geometrid species. However, a final estimate of resolution success awaits detailed taxonomic analysis of 48 species where patterns of barcode variation suggest cases of cryptic species, unrecognized synonymy as well as young species. A catalog of these taxa meriting further taxonomic investigation is presented as well as the supplemental information needed to facilitate these investigations.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0018290