Composition of gut microbiota in patients with toxigenic Clostridioides

Data concerning the human microbiota composition during Clostridioides (Clostridium) difficile infection (CDI) using next-generation sequencing are still limited. We aimed to confirm key features indicating tcdB positive patients and compare the microbiota composition between subgroups based on toxi...

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Bibliographic Details
Published in:PloS one 2019-02, Vol.14 (2), p.e0212626
Main Authors: Han, Sung-Hee, Yi, Joowon, Kim, Ji-Hoon, Lee, SangWon, Moon, Hee-Won
Format: Article
Language:English
Subjects:
Antibiotics
Clostridium infections
Complications and side effects
Diagnosis
Diarrhea
Genes
Infection
Microbiota (Symbiotic organisms)
RNA
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Summary:Data concerning the human microbiota composition during Clostridioides (Clostridium) difficile infection (CDI) using next-generation sequencing are still limited. We aimed to confirm key features indicating tcdB positive patients and compare the microbiota composition between subgroups based on toxin gene load (tcdB gene) and presence of significant diarrhea. Ninety-nine fecal samples from 79 tcdB positive patients and 20 controls were analyzed using 16S rRNA gene sequencing. Chao1 index for alpha diversity were calculated and principal coordinate analysis was performed for beta diversity using Quantitative Insights into Microbial Ecology (QIIME) pipeline. The mean relative abundance in each group was compared at phylum, family, and genus levels. There were significant alterations in alpha and beta diversity in tcdB positive patients (both colonizer and CDI) compared with those in the control. The mean Chao1 index of tcdB positive patients was significantly lower than the control group (P
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0212626