Interaction of YAP with the Myb-MuvB

The Hippo signalling pathway and its central effector YAP regulate proliferation of cardiomyocytes and growth of the heart. Using genetic models in mice we show that the increased proliferation of embryonal and postnatal cardiomyocytes due to loss of the Hippo-signaling component SAV1 depends on the...

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Bibliographic Details
Published in:PLoS genetics 2020-05, Vol.16 (5)
Main Authors: Gründl, Marco, Walz, Susanne, Hauf, Laura, Schwab, Melissa, Werner, Kerstin Marcela, Spahr, Susanne, Schulte, Clemens, Maric, Hans Michael, Ade, Carsten P, Gaubatz, Stefan
Format: Article
Language:English
Subjects:
Cell proliferation
Genetic aspects
Genetic research
Heart cells
Physiological aspects
Transcription (Genetics)
Transcriptional coactivators
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Summary:The Hippo signalling pathway and its central effector YAP regulate proliferation of cardiomyocytes and growth of the heart. Using genetic models in mice we show that the increased proliferation of embryonal and postnatal cardiomyocytes due to loss of the Hippo-signaling component SAV1 depends on the Myb-MuvB (MMB) complex. Similarly, proliferation of postnatal cardiomyocytes induced by constitutive active YAP requires MMB. Genome studies revealed that YAP and MMB regulate an overlapping set of cell cycle genes in cardiomyocytes. Protein-protein interaction studies in cell lines and with recombinant proteins showed that YAP binds directly to B-MYB, a subunit of MMB, in a manner dependent on the YAP WW domains and a PPXY motif in B-MYB. Disruption of the interaction by overexpression of the YAP binding domain of B-MYB strongly inhibits the proliferation of cardiomyocytes. Our results point to MMB as a critical downstream effector of YAP in the control of cardiomyocyte proliferation.
ISSN:1553-7390
1553-7404
DOI:10.1371/journal.pgen.1008818