Effect of cadmium stress on certain physiological parameters, antioxidative enzyme activities and biophoton emission of leaves in barley

• Published in: PloS one 2020-11, Vol.15 (11), p.e0240470
• Main Authors: Jocsak, Ildiko, Malgwi, Isaac, Rabnecz, Gyula, Szego, Anita, Varga-Visi, Eva, Vegvari, Gyorgy, Pónya, Zsolt
• Format: Article
• Language: English
• Subjects: Barley; Biosynthesis; Botanical research; Cadmium; Enzyme activation; Photons; Physiological aspects; Seedlings; Stress (Physiology)
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0240470

Biophoton emission is a well-known phenomenon in living organisms, including plant species; however, the underlying mechanisms are not yet well elucidated. Nevertheless, non-invasive stress detection is of high importance when in plant production and plant research. Therefore, the aim of our work was to investigate, whether biophoton emission is suitable for the detection of cadmium stress in the early phase of stress evolution and to identify certain stress-related events that occur rapidly upon cadmium exposure of barley seedlings parallel to biophoton emission measurements. Changes of biophoton emission, chlorophyll content estimation index, ascorbate level, the activity of ascorbate- and guaiacol peroxidase enzymes and lipid oxidation were measured during seven days of cadmium treatment in barley (Hordeum vulgare L.) seedlings. The results indicate that the antioxidant enzyme system responded the most rapidly to the stress caused by cadmium and the lipid oxidation-related emission of photons was detected in cadmium-treated samples as early as one day after cadmium exposure. Furthermore, a concentration-dependent increase in biophoton emission signals indicating an increased rate of antioxidative enzymes and lipid oxidation was also possible to determine. Our work shows evidence that biophoton emission is suitable to identify the initial phase of cadmium stress effectively and non-invasively.