Comparison of chemical-induced temporomandibular osteoarthritis rat models

• Published in: PloS one 2023-01, Vol.18 (1), p.e0281135
• Main Authors: Barry, Florent, Chai, Feng, Chijcheapaza-Flores, Henry, Garcia-Fernandez, Maria José, Blanchemain, Nicolas, Nicot, Romain
• Format: Article
• Language: English
• Subjects: Antiarthritic agents; Care and treatment; Complications and side effects; Drug delivery systems; Drugs; Evaluation; Patient outcomes; Temporomandibular joint disorders; Vehicles
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0281135

To compare two agents that can induce a rat model of temporomandibular joint osteoarthritis (TMJOA) by chemical induction: monosodium iodoacetate (MIA) and collagenase type 2 (Col-2). We wished to ascertain the best agent for assessing drug-delivery systems (DDSs). Male Wistar rats underwent intra-articular injection with MIA or Col-2. They were manipulated for 30 days. The head withdrawal threshold (HWT), immunohistological assessment, and positron emission tomography (PET) were used to evaluate the relevance of our models. For both the MIA and Col-2 groups, pain persisted for 30 days after injection. Change in the HWT showed that Col-2 elicited a strong action initially that decreased progressively. MIA had a constant action upon pain behavior. Histology of TMJ tissue from both groups showed progressive degradation of TMJ components. MIA and Col-2 induced orofacial pain by their local chemical action on TMJs. However, based on a prolonged and greater sustained effect on the pain threshold, persistent histological changes, and imaging results, MIA appeared to be more suitable for creation of a rat model of TMJOA for the study of DDSs.