Molecular characterization, virulence and antimicrobial and biocidal susceptibility of selected bacteria isolated from the cloaca of nestling ospreys

In the present study, the presence of the Enterobacterales, Staphylococcus spp., Mammaliicoccus spp., and Enterococcus spp. in cloacal samples of nestling ospreys (Pandion haliaetus), a fish-eating specialist, from Mono Lake, California, USA was examined by a multiphasic approach, including antimicr...

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Published in:PloS one 2024-09, Vol.19 (9), p.e0311306
Main Authors: Loncaric, Igor, Szostak, Michael P, Cabal-Rosel, Adriana, Grünzweil, Olivia M, Riegelnegg, Alina, Misic, Dusan, Müller, Elke, Feßler, Andrea T, Braun, Sascha D, Schwarz, Stefan, Monecke, Stefan, Ehricht, Ralf, Ruppitsch, Werner, Spergser, Joachim, Lewis, Ashli, Bloom, Peter H, Saggese, Miguel D
Format: Article
Language:English
Subjects:
Birds
Diseases
Drug resistance in microorganisms
Drug therapy
Enterobacteriaceae infections
Environmental monitoring
Methods
Nestlings
Ospreys
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Summary:In the present study, the presence of the Enterobacterales, Staphylococcus spp., Mammaliicoccus spp., and Enterococcus spp. in cloacal samples of nestling ospreys (Pandion haliaetus), a fish-eating specialist, from Mono Lake, California, USA was examined by a multiphasic approach, including antimicrobial and biocide susceptibility testing, genotyping, and whole genome sequencing of selected isolates. The most commonly detected species was Escherichia coli, followed by Mammaliicoccus sciuri, Staphylococcus delphini, Enterococcus faecalis, Enterococcus faecium, Hafnia alvei, Klebsiella pneumoniae, Citrobacter braakii and single isolates of Edwardsiella tarda, Edwardsiella albertii, Klebsiella aerogenes, Plesiomonas shigelloides and Staphylococcus pseudintermedius. Multi-drug resistance (MDR) was observed in two E. coli isolates and in an Enterococcus faecium isolate. The MDR bla.sub.CTX-M-55 -positive E. coli belonged to the pandemic clone ST58. The results of the present study suggest that nestling ospreys are exposed to MDR bacteria, possibly through the ingestion of contaminated fish. Ospreys may be good biosentinels for the presence of these microorganisms and antibiotic resistance in the local environment and the risk for other wildlife, livestock and humans.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0311306