Determination of 7B3 Residues in Cotton Plants by Liquid Chromatography/Mass Spectrometry

A method was developed for the determination of 7B3 (12-propyloxyimino-1,15-pentadecanlactam), a novel macrolactam fungicide, by liquid chromatography/mass spectrometry (LC/MS) with positive electrospray ionization (ESI+). The method used a reversed-phase C18 column and acetonitrile-water (60 + 40,...

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Bibliographic Details
Published in:Journal of AOAC International 2009, Vol.92 (1), p.302-306
Main Authors: Yan, Xiao-Jing, Liang, Xiao-Mei, Xu, Yan-Jun, Jin, Shu-Hui, Wang, Dao-Quan
Format: Article
Language:English
Subjects:
accuracy
calibration
Centrifugation
chemical structure
Chromatography, Liquid - methods
cotton
fungicide residues
fungicides
Fungicides, Industrial - analysis
Gossypium
Gossypium - chemistry
Identification and classification
Indicators and Reagents
Lactams - analysis
Liquid chromatography
Mass spectrometry
Mass Spectrometry - methods
maximum residue limits
Methods
Pesticides
quantitative analysis
rapid methods
Solutions
Spectrometry, Mass, Electrospray Ionization - methods
validity
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Summary:A method was developed for the determination of 7B3 (12-propyloxyimino-1,15-pentadecanlactam), a novel macrolactam fungicide, by liquid chromatography/mass spectrometry (LC/MS) with positive electrospray ionization (ESI+). The method used a reversed-phase C18 column and acetonitrile-water (60 + 40, v/v) mobile phase. The quick, easy, cheap, effective, rugged, and safe method was used for extraction of 7B3 from cotton plants, which involved the extraction of 10 g homogenized sample with 10 mL acetonitrile, followed by the addition of 4 g anhydrous MgSO4 and 1.0 g NaCl. After centrifugation, 1 mL of the buffered acetonitrile extract was transferred into a tube containing 50 mg primary secondary amine sorbent and 100 mg anhydrous MgSO4. After shaking and centrifugation, the final extract was transferred to an autosampler vial for concurrent analysis by LC/MS. The results of 7B3 determined by LC/MS in the selective ion monitoring mode were linear, and the matrix effect of the method was evaluated. The average recoveries of 7B3 fortified at different levels were within 84.1-100.2%, and the relative standard deviations were
ISSN:1060-3271
1944-7922
DOI:10.1093/jaoac/92.1.302