Hepatozoon canis infection in a domestic dog in Southern Brazil confirmed by molecular techniques/Infeccao por Hepatozoon canis em canino domestico na regiao Sul do Brasil confirmada por tecnicas moleculares

Canine hepatozoonosis is a vector-borne disease caused by the protozoa Hepatozoon spp. There are few case reports in Brazil and the epidemiology, pathogenicity, vectors and molecular characterization is poorly understood. The present study used a canine presenting weight loss, regenerative anemia, n...

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Bibliographic Details
Published in:Ciência rural 2009-10, Vol.39 (7), p.2135
Main Authors: Lasta, Camila Serina, dos Santos, Andrea Pires, Mello, Fabiola Peixoto da Silva, Lacerda, Luciana de Almeida, Messick, Joanne Belle, Gonzalez, Felix Hilario Diaz
Format: Article
Language:Spanish
Subjects:
Anemia
DNA
Epidemiology
Health aspects
Methods
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Summary:Canine hepatozoonosis is a vector-borne disease caused by the protozoa Hepatozoon spp. There are few case reports in Brazil and the epidemiology, pathogenicity, vectors and molecular characterization is poorly understood. The present study used a canine presenting weight loss, regenerative anemia, neutropenia and hyperglobulinemia. Hepatozoon spp. gamonts were observed in the blood smear by microscopy. DNA was extracted from blood, and the diagnosis was confirmed by PCR assay. The PCR product was purified from gel and cloned. A fragment of 625bp was sequenced and submitted to the GenBank database as Isolate Porto Alegre. Molecular analysis showed a homology of 98-100% with Hepatozoon canis and less than 92% for H. americanum. This study represents the first molecular confirmation that H. canis is present in Southern Brazil. Key words: Hepatozoon canis, hemoparasite, dog, PCR, Brazil. Hepatozoonose canina e uma doenca transmitida por carrapatos e causada pelo protozoario Hepatozoon spp. No Brasil, existem poucos relatos da infeccao e dados sobre sua epidemiologia, patogenicidade, seus vetores e sua caracterizacao genetica. No presente estudo, sao utilizadas tecnicas moleculares para o diagnostico e a caracterizacao do parasita. Foi estudado um canino que apresentava emagrecimento progressivo, anemia regenerativa, neutropenia e hiperglobulinemia. Gamontes de Hepatozoon spp. foram visualizados no esfregaco sanguineo. DNA foi extraido do sangue, e o diagnostico foi confirmado pela tecnica da reacao em cadeia da polimerase (PCR). O produto da PCR foi purificado a partir do gel e clonado, e o fragmento de 625pb foi sequenciado. A sequencia foi submetida ao GenBank como isolado de Porto Alegre, e a analise molecular revelou homologia de 98-100% com Hepatozoon canis e maxima de 92% com Hepatozoon americanum. Este estudo representa a primeira confirmacao molecular da presenca de H. canis no sul do Brasil. Palavras-chave: Hepatozoon canis, hemoparasita, cao, PCR, Brasil.
ISSN:0103-8478