Using the serological technique to identify Ascia monuste orseis

• Published in: Acta Scientiarum. Biological Sciences (UEM) 2009, Vol.31 (2), p.149
• Main Authors: Costa, Fabiano de Mello, Loffredo, Ana Paula da Silva, Onody, Helena Caroline, Sousa-Silva, Carlos Roberto
• Format: Report
• Language: Spanish
• Subjects: Biological control; Pests
• ISSN: 1679-9283
• DOI: 10.4025/actascibiolsci.v31i2.515

This study aimed to obtain specific antiserum for Ascia monuste orseis (Latreille, 1819), with the aim of identifying the predators associated with this insect. The preparation of specific antiserum was performed by immunizing a rabbit via a lymph nodule injection containing antigen obtained from the maceration of 4th and 5th instars of A. monuste orseis caterpillars. Serological homologous tests performed using double diffusion in agar gel were positive 21 days after the first antigen inoculation. Eggs, 1th, 2th and 3th instar caterpillars, pupae and adults of A. monuste orseis also reacted positively to the obtained antiserum. Key words: Insecta, biological control, antiserum, predation. O presente trabalho teve como objetivo obter antissoro especifico para Ascia monuste orseis (Latreille, 1819) com a finalidade de identificar seus predadores. Para a obtencao do antissoro, um coelho foi imunizado, via linfonodulo, com uma injecao de antigeno obtido pela maceracao de lagartas do 4 e 5 instares de A. monuste orseis. Reacoes serologicas homologas, realizadas por meio do metodo de dupla difusao em agar, foram positivas apos 21 dias da 1a inoculacao do antigeno. Ovos, lagartas do 1, 2 e 3 instares, pupas e adultos de A. monuste orseis tambem reagiram positivamente ao antissoro obtido. Palavras-chave: Insecta, controle biologico, antissoros, predacao.