Simultaneous Determination of Residual Thiamphenicol and Florfenicol in Foods of Animal Origin by HPLC/Electrospray Ionization-MS/MS

A simple and reliable method using HPLC/MS/MS has been developed for determination of thiamphenicol and florfenicol in foods of animal origin, such as pork, porcine liver, porcine kidney, beef, bovine liver, fish, and chicken. The HPLC separation was performed on a Waters XTerra phenyl column (100 x...

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Bibliographic Details
Published in:Journal of AOAC International 2009-07, Vol.92 (4), p.1225-1232
Main Authors: Chou, Kuang Yu, Cheng, Tsung Yao, Chen, Ching Mei, Hung, Pei LIng, Tang, Yu Yun, Chung-Wang, Yueh Jong, Shih, Yang-Chih
Format: Article
Language:English
Subjects:
animal-based foods
Animals
Anti-Bacterial Agents - analysis
antibiotic residues
Antibiotics
beef
beef cattle
Biological and medical sciences
Cattle
Chemical properties
chemical structure
Chickens
chloramphenicol
Chromatography, High Pressure Liquid
Composition
Drug Residues
fish
Fishes
florfenicol
Food
Food industries
Fundamental and applied biological sciences. Psychology
High performance liquid chromatography
Identification and classification
Indicators and Reagents
kidneys as food
livers as food
Mass spectrometry
Meat - analysis
Methods
quantitative analysis
reversed-phase high performance liquid chromatography
Solutions
Spectrometry, Mass, Electrospray Ionization
Swine
Thiamphenicol - analogs & derivatives
Thiamphenicol - analysis
veterinary drugs
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Summary:A simple and reliable method using HPLC/MS/MS has been developed for determination of thiamphenicol and florfenicol in foods of animal origin, such as pork, porcine liver, porcine kidney, beef, bovine liver, fish, and chicken. The HPLC separation was performed on a Waters XTerra phenyl column (100 x 2.1 mm, 3.5 μm) with a gradient mobile phase system of 0.1% formic acid-methanol at a flow rate 0.35 mL/min. Negative ionization produced the molecular ions at m/z 354 and 356 for thiamphenicol and florfenicol, respectively. Two characteristic transition reactions (m/z 354 leads to 185, 290 and m/z 356 leads to 336, 185) in the selected reaction monitoring mode were tested for detection and confirmation of thiamphenicol and florfenicol simultaneously. The analytes were extracted with ethyl acetate and defatted with n-hexane by liquid-liquid extraction. The recoveries of thiamphenicol and florfenicol by the developed method were 72.5-97.6%. The limits of determination were 1.0 ng/g (ppb) for both compounds.
ISSN:1060-3271
1944-7922
DOI:10.1093/jaoac/92.4.1225