Determination of the Metrological Characteristics of the Near-Field Scanning Optical Microscope in the Study of Biological Objects

Methods of determining the basic metrological characteristics of a near-field scanning optical microscope, i.e., scale factor and spatial resolution, defined as the diameter of the optical probe, are proposed. The use of the proposed method for calibration of the near-field scanning optical microsco...

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Bibliographic Details
Published in:Measurement techniques 2014, Vol.56 (10), p.1173-1180
Main Authors: Bulygin, F. V., Dracheva, O. E., Kutuzov, N. P., Lyaskovskii, V. L., Maksimov, G. V., Nikolaev, Yu. A.
Format: Article
Language:English
Subjects:
Analytical Chemistry
Characterization and Evaluation of Materials
Measurement Science and Instrumentation
Medical and Biological Measurements
Nuclear radiation
Physical Chemistry
Physics
Physics and Astronomy
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Summary:Methods of determining the basic metrological characteristics of a near-field scanning optical microscope, i.e., scale factor and spatial resolution, defined as the diameter of the optical probe, are proposed. The use of the proposed method for calibration of the near-field scanning optical microscope directly in the course of measurements of the parameters of biological objects is investigated.
ISSN:0543-1972
1573-8906
DOI:10.1007/s11018-014-0351-1