Fluorescence quenching study of the effect of association on the structure of humic acids

Using as an example the concentration dependence of the fluorescence quenching constants for quenching by Cd 2+ ions for different weight fractions of humic acid, we have demonstrated the application of the fluorescence quenching method for studying the effect of association on the structure of humi...

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Bibliographic Details
Published in:Journal of applied spectroscopy 2010-09, Vol.77 (4), p.582-587
Main Authors: Lavrik, N. L., Mulloev, N. U.
Format: Article
Language:English
Subjects:
Analytical Chemistry
Atomic/Molecular Structure and Spectra
Fluorescence
Humic acid
Physics
Physics and Astronomy
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Summary:Using as an example the concentration dependence of the fluorescence quenching constants for quenching by Cd 2+ ions for different weight fractions of humic acid, we have demonstrated the application of the fluorescence quenching method for studying the effect of association on the structure of humic acids. We have established that the quenching constants for the light fraction (5–30 kD) decrease as the humic acid concentration increases and the quenching constants increase for the heavy fraction (60–100 kD). The observed dependences are interpreted in terms of the idea of oppositely directed effects of association on the structure of the environment of fluorophores located within the core and peripheral regions of the humic acid macromolecule.
ISSN:0021-9037
1573-8647
DOI:10.1007/s10812-010-9372-y