Loading…

The correlation of Tim-3 and IFN-γ expressions in mice infected with Toxoplasma gondii during gestation

The immunoinhibitory receptor T cell immunoglobulin domain and mucin domain-1 (Tim-1) and Tim-3 participate in the regulation of Th immune response as well as innate immunity. However, there is no report about the expression of Tim genes in Toxoplasma gondii-infected experimental models during pregn...

Full description

Saved in:

Bibliographic Details
Published in:	Parasitology Research 2015, p.125
Main Authors:	Fu, Xiaoyin, Wu, Bin, Huang, Bo, Zheng, Huanqin, Huang, Shiguang, Gan, Yan, Shen, Jilong, Lun, Zhao-Rong, Lu, Fangli
Format:	Report
Language:	English
Subjects:	Health aspects Host-bacteria relationships Identification and classification Immunoglobulins Interferon Pregnancy T cells Toxoplasma
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

cited_by
cites
container_end_page
container_issue
container_start_page	125
container_title	Parasitology Research
container_volume
creator	Fu, Xiaoyin Wu, Bin Huang, Bo Zheng, Huanqin Huang, Shiguang Gan, Yan Shen, Jilong Lun, Zhao-Rong Lu, Fangli
description	The immunoinhibitory receptor T cell immunoglobulin domain and mucin domain-1 (Tim-1) and Tim-3 participate in the regulation of Th immune response as well as innate immunity. However, there is no report about the expression of Tim genes in Toxoplasma gondii-infected experimental models during pregnancy. In this study, Kunming outbred pregnant mice were infected with RH strain of T gondii through vagina at days 10 to 16 of gestation, and the mRNA expressions of Tim-1, Tim-3, interleukin (IL)-4, and interferon (IFN)-γ in the placentas, uteri, and draining lumber aortic lymph nodes (LALNs) at day 18 of gestation were analyzed using quantitative real-time PCR (qRT-PCR). Compared with uninfected pregnant controls, significantly increased levels of IFN-γ and Tim-3 were detected in the placentas (P
doi_str_mv	10.1007/s00436-014-4167-3
format	report
fullrecord	<record><control><sourceid>gale</sourceid><recordid>TN_cdi_gale_infotracacademiconefile_A407528078</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A407528078</galeid><sourcerecordid>A407528078</sourcerecordid><originalsourceid>FETCH-gale_infotracacademiconefile_A4075280783</originalsourceid><addsrcrecordid>eNqVjEFOwzAQRb0AqS3tAdjNBVzGtRuXJUKtYMMq-8qKJ8mgxK5sI3ov7sGZsBAXQH_xpP_1nxD3CrcK0T5kRKMbicpIoxor9Y1Y4qPe1UbphVjl_I6obGPMUoztSNDFlGhyhWOA2EPLs9TggofX05v8_gK6XhLlXOcMHGDmjip76gp5-OQyQhuv8TK5PDsYYvDM4D8ShwEGyuVXvBa3vZsybf54J7anY_v8Igc30bnaYkmuq_FU_TFQz7V_Mmj3uwPag_734QfEKVTl</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>report</recordtype></control><display><type>report</type><title>The correlation of Tim-3 and IFN-γ expressions in mice infected with Toxoplasma gondii during gestation</title><source>Springer Nature:Jisc Collections:Springer Nature Read and Publish 2023-2025: Springer Reading List</source><creator>Fu, Xiaoyin ; Wu, Bin ; Huang, Bo ; Zheng, Huanqin ; Huang, Shiguang ; Gan, Yan ; Shen, Jilong ; Lun, Zhao-Rong ; Lu, Fangli</creator><creatorcontrib>Fu, Xiaoyin ; Wu, Bin ; Huang, Bo ; Zheng, Huanqin ; Huang, Shiguang ; Gan, Yan ; Shen, Jilong ; Lun, Zhao-Rong ; Lu, Fangli</creatorcontrib><description>The immunoinhibitory receptor T cell immunoglobulin domain and mucin domain-1 (Tim-1) and Tim-3 participate in the regulation of Th immune response as well as innate immunity. However, there is no report about the expression of Tim genes in Toxoplasma gondii-infected experimental models during pregnancy. In this study, Kunming outbred pregnant mice were infected with RH strain of T gondii through vagina at days 10 to 16 of gestation, and the mRNA expressions of Tim-1, Tim-3, interleukin (IL)-4, and interferon (IFN)-γ in the placentas, uteri, and draining lumber aortic lymph nodes (LALNs) at day 18 of gestation were analyzed using quantitative real-time PCR (qRT-PCR). Compared with uninfected pregnant controls, significantly increased levels of IFN-γ and Tim-3 were detected in the placentas (P<0.001), uteri (P=0.003 and P=0.017, respectively), and LALNs (P=0.003 and P=0.025, respectively) of T. gondii-infected mice; there were positive and significant correlations between Tim-3 and IFN-γ mRNA expression levels in the placentas ([R.sup.2] = 0.6331, P = 0.0011), uteri ([R.sup.2] = 0.5658, P=0.003), and LALNs ([R.sup.2] = 0.5583, P=0.0033) of infected mice. Tim-1 (P=0.002) and IL-4 (P=0.003) expressions were significantly increased in the placentas, but Tim-1 were significantly decreased in the uteri (P=0.013) and LALNs (P<0.001) of infected pregnant mice in comparison of uninfected pregnant controls. Our data suggested that Tim3 may play a regulatory role in T gondii-infected pregnant mouse model. Keywords Toxoplasma gondii * Mice * Pregnancy * IFN-γ * Tim-3</description><identifier>ISSN: 0932-0113</identifier><identifier>DOI: 10.1007/s00436-014-4167-3</identifier><language>eng</language><publisher>Springer</publisher><subject>Health aspects ; Host-bacteria relationships ; Identification and classification ; Immunoglobulins ; Interferon ; Pregnancy ; T cells ; Toxoplasma</subject><ispartof>Parasitology Research, 2015, p.125</ispartof><rights>COPYRIGHT 2015 Springer</rights><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>776,780,4476,27902</link.rule.ids></links><search><creatorcontrib>Fu, Xiaoyin</creatorcontrib><creatorcontrib>Wu, Bin</creatorcontrib><creatorcontrib>Huang, Bo</creatorcontrib><creatorcontrib>Zheng, Huanqin</creatorcontrib><creatorcontrib>Huang, Shiguang</creatorcontrib><creatorcontrib>Gan, Yan</creatorcontrib><creatorcontrib>Shen, Jilong</creatorcontrib><creatorcontrib>Lun, Zhao-Rong</creatorcontrib><creatorcontrib>Lu, Fangli</creatorcontrib><title>The correlation of Tim-3 and IFN-γ expressions in mice infected with Toxoplasma gondii during gestation</title><title>Parasitology Research</title><description>The immunoinhibitory receptor T cell immunoglobulin domain and mucin domain-1 (Tim-1) and Tim-3 participate in the regulation of Th immune response as well as innate immunity. However, there is no report about the expression of Tim genes in Toxoplasma gondii-infected experimental models during pregnancy. In this study, Kunming outbred pregnant mice were infected with RH strain of T gondii through vagina at days 10 to 16 of gestation, and the mRNA expressions of Tim-1, Tim-3, interleukin (IL)-4, and interferon (IFN)-γ in the placentas, uteri, and draining lumber aortic lymph nodes (LALNs) at day 18 of gestation were analyzed using quantitative real-time PCR (qRT-PCR). Compared with uninfected pregnant controls, significantly increased levels of IFN-γ and Tim-3 were detected in the placentas (P<0.001), uteri (P=0.003 and P=0.017, respectively), and LALNs (P=0.003 and P=0.025, respectively) of T. gondii-infected mice; there were positive and significant correlations between Tim-3 and IFN-γ mRNA expression levels in the placentas ([R.sup.2] = 0.6331, P = 0.0011), uteri ([R.sup.2] = 0.5658, P=0.003), and LALNs ([R.sup.2] = 0.5583, P=0.0033) of infected mice. Tim-1 (P=0.002) and IL-4 (P=0.003) expressions were significantly increased in the placentas, but Tim-1 were significantly decreased in the uteri (P=0.013) and LALNs (P<0.001) of infected pregnant mice in comparison of uninfected pregnant controls. Our data suggested that Tim3 may play a regulatory role in T gondii-infected pregnant mouse model. Keywords Toxoplasma gondii * Mice * Pregnancy * IFN-γ * Tim-3</description><subject>Health aspects</subject><subject>Host-bacteria relationships</subject><subject>Identification and classification</subject><subject>Immunoglobulins</subject><subject>Interferon</subject><subject>Pregnancy</subject><subject>T cells</subject><subject>Toxoplasma</subject><issn>0932-0113</issn><fulltext>true</fulltext><rsrctype>report</rsrctype><creationdate>2015</creationdate><recordtype>report</recordtype><sourceid/><recordid>eNqVjEFOwzAQRb0AqS3tAdjNBVzGtRuXJUKtYMMq-8qKJ8mgxK5sI3ov7sGZsBAXQH_xpP_1nxD3CrcK0T5kRKMbicpIoxor9Y1Y4qPe1UbphVjl_I6obGPMUoztSNDFlGhyhWOA2EPLs9TggofX05v8_gK6XhLlXOcMHGDmjip76gp5-OQyQhuv8TK5PDsYYvDM4D8ShwEGyuVXvBa3vZsybf54J7anY_v8Igc30bnaYkmuq_FU_TFQz7V_Mmj3uwPag_734QfEKVTl</recordid><startdate>20150101</startdate><enddate>20150101</enddate><creator>Fu, Xiaoyin</creator><creator>Wu, Bin</creator><creator>Huang, Bo</creator><creator>Zheng, Huanqin</creator><creator>Huang, Shiguang</creator><creator>Gan, Yan</creator><creator>Shen, Jilong</creator><creator>Lun, Zhao-Rong</creator><creator>Lu, Fangli</creator><general>Springer</general><scope/></search><sort><creationdate>20150101</creationdate><title>The correlation of Tim-3 and IFN-γ expressions in mice infected with Toxoplasma gondii during gestation</title><author>Fu, Xiaoyin ; Wu, Bin ; Huang, Bo ; Zheng, Huanqin ; Huang, Shiguang ; Gan, Yan ; Shen, Jilong ; Lun, Zhao-Rong ; Lu, Fangli</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-gale_infotracacademiconefile_A4075280783</frbrgroupid><rsrctype>reports</rsrctype><prefilter>reports</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Health aspects</topic><topic>Host-bacteria relationships</topic><topic>Identification and classification</topic><topic>Immunoglobulins</topic><topic>Interferon</topic><topic>Pregnancy</topic><topic>T cells</topic><topic>Toxoplasma</topic><toplevel>online_resources</toplevel><creatorcontrib>Fu, Xiaoyin</creatorcontrib><creatorcontrib>Wu, Bin</creatorcontrib><creatorcontrib>Huang, Bo</creatorcontrib><creatorcontrib>Zheng, Huanqin</creatorcontrib><creatorcontrib>Huang, Shiguang</creatorcontrib><creatorcontrib>Gan, Yan</creatorcontrib><creatorcontrib>Shen, Jilong</creatorcontrib><creatorcontrib>Lun, Zhao-Rong</creatorcontrib><creatorcontrib>Lu, Fangli</creatorcontrib></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Fu, Xiaoyin</au><au>Wu, Bin</au><au>Huang, Bo</au><au>Zheng, Huanqin</au><au>Huang, Shiguang</au><au>Gan, Yan</au><au>Shen, Jilong</au><au>Lun, Zhao-Rong</au><au>Lu, Fangli</au><format>book</format><genre>unknown</genre><ristype>RPRT</ristype><atitle>The correlation of Tim-3 and IFN-γ expressions in mice infected with Toxoplasma gondii during gestation</atitle><jtitle>Parasitology Research</jtitle><date>2015-01-01</date><risdate>2015</risdate><spage>125</spage><pages>125-</pages><issn>0932-0113</issn><abstract>The immunoinhibitory receptor T cell immunoglobulin domain and mucin domain-1 (Tim-1) and Tim-3 participate in the regulation of Th immune response as well as innate immunity. However, there is no report about the expression of Tim genes in Toxoplasma gondii-infected experimental models during pregnancy. In this study, Kunming outbred pregnant mice were infected with RH strain of T gondii through vagina at days 10 to 16 of gestation, and the mRNA expressions of Tim-1, Tim-3, interleukin (IL)-4, and interferon (IFN)-γ in the placentas, uteri, and draining lumber aortic lymph nodes (LALNs) at day 18 of gestation were analyzed using quantitative real-time PCR (qRT-PCR). Compared with uninfected pregnant controls, significantly increased levels of IFN-γ and Tim-3 were detected in the placentas (P<0.001), uteri (P=0.003 and P=0.017, respectively), and LALNs (P=0.003 and P=0.025, respectively) of T. gondii-infected mice; there were positive and significant correlations between Tim-3 and IFN-γ mRNA expression levels in the placentas ([R.sup.2] = 0.6331, P = 0.0011), uteri ([R.sup.2] = 0.5658, P=0.003), and LALNs ([R.sup.2] = 0.5583, P=0.0033) of infected mice. Tim-1 (P=0.002) and IL-4 (P=0.003) expressions were significantly increased in the placentas, but Tim-1 were significantly decreased in the uteri (P=0.013) and LALNs (P<0.001) of infected pregnant mice in comparison of uninfected pregnant controls. Our data suggested that Tim3 may play a regulatory role in T gondii-infected pregnant mouse model. Keywords Toxoplasma gondii * Mice * Pregnancy * IFN-γ * Tim-3</abstract><pub>Springer</pub><doi>10.1007/s00436-014-4167-3</doi></addata></record>
fulltext	fulltext
identifier	ISSN: 0932-0113
ispartof	Parasitology Research, 2015, p.125
issn	0932-0113
language	eng
recordid	cdi_gale_infotracacademiconefile_A407528078
source	Springer Nature:Jisc Collections:Springer Nature Read and Publish 2023-2025: Springer Reading List
subjects	Health aspects Host-bacteria relationships Identification and classification Immunoglobulins Interferon Pregnancy T cells Toxoplasma
title	The correlation of Tim-3 and IFN-γ expressions in mice infected with Toxoplasma gondii during gestation
url	http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-22T10%3A21%3A49IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale&rft_val_fmt=info:ofi/fmt:kev:mtx:book&rft.genre=unknown&rft.atitle=The%20correlation%20of%20Tim-3%20and%20IFN-%CE%B3%20expressions%20in%20mice%20infected%20with%20Toxoplasma%20gondii%20during%20gestation&rft.jtitle=Parasitology%20Research&rft.au=Fu,%20Xiaoyin&rft.date=2015-01-01&rft.spage=125&rft.pages=125-&rft.issn=0932-0113&rft_id=info:doi/10.1007/s00436-014-4167-3&rft_dat=%3Cgale%3EA407528078%3C/gale%3E%3Cgrp_id%3Ecdi_FETCH-gale_infotracacademiconefile_A4075280783%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_id=info:pmid/&rft_galeid=A407528078&rfr_iscdi=true