H.sub.2S acting as a downstream signaling molecule of SA regulates Cd tolerance in Arabidopsis

• Published in: Plant and soil 2015-08, Vol.393 (1-2), p.137
• Main Authors: Qiao, Zengjie, Jing, Tao, Liu, Zhiqiang, Zhang, Liping, Jin, Zhuping, Liu, Danmei, Pei, Yanxi
• Format: Article
• Language: English
• Subjects: Arabidopsis thaliana; Health aspects; Photosynthesis; Signaling peptides and proteins
• ISSN: 0032-079X
• DOI: 10.1007/s11104-015-2475-8

Background and aims In plants, salicylic acid (SA) acting as one of the signaling molecules can regulate and respond to cadmium (Cd) stress. It is well known that hydrogen sulfide (H.sub.2S) could alleviate toxicity of Cd stress but the crosstalk between SA and H.sub.2S in regulating Cd stress still remains unclear. Methods We studied on it through the physiological and biochemical method together with the microstructure synthesis. Results Our results indicated that the plants exhibited enhanced tolerance to Cd when pretreated with SA, which included weakening oxidative damage and intensifying photosynthesis as well as boosting L-cysteine desulfhydrase (LCD) activity and raising the content of endogenous H.sub.2S. In parallel, the plants pretreated with NaHS, a donor of H.sub.2S, showed a stronger ability to tolerate Cd stress, SA- pretreated plants presented normal growth and meanwhile the content of chlorophyll and the microstructure of roots were significantly different compared to those treated with Cd only. By contrast, with the same treatments, the positive function and effect of SA on the LCD-knockout mutants, lcd was not observed. Pharmacological experiments further testified these results. Conclusions All of the results suggest that H.sub.2S may be a downstream signal molecule in SA-induced Cd tolerance of Arabidopsis. Electronic supplementary material The online version of this article (doi:10.1007/s11104-015-2475-8) contains supplementary material, which is available to authorized users.