Free fatty acids profile among lean, overweight and obese non-alcoholic fatty liver disease patients: a case - control study

Background Non-alcoholic fatty liver disease (NAFLD) given its association with obesity and diabetes may perhaps exert distinct free fatty acids (FFA) pattern, but the understanding of this phenomenon is limited. To this effect, we evaluated FFA profiles among healthy subjects and NAFLD patients str...

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Published in:Lipids in Health and Disease 2017, Vol.16 (1)
Main Authors: Feng, Rennan, Luo, Chao, Li, Chunlong, Du, Shanshan, Okekunle, Akinkunmi Paul, Li, Yanchuan, Chen, Yang, Zi, Tianqi, Niu, Yucun
Format: Report
Language:English
Subjects:
Body weight
Care and treatment
Diabetes mellitus
Diagnosis
Health aspects
Liver diseases
Mass spectrometry
Obesity
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Summary:Background Non-alcoholic fatty liver disease (NAFLD) given its association with obesity and diabetes may perhaps exert distinct free fatty acids (FFA) pattern, but the understanding of this phenomenon is limited. To this effect, we evaluated FFA profiles among healthy subjects and NAFLD patients stratified by body weight, to identify FFA valuable for early diagnosis of NAFLD. Methods Serum FFA profiles of healthy and NAFLD (lean, overweight and obese) subjects was determined using gas chromatography-mass spectrometry (GC-MS) and distinctions in FFA patterns were evaluated using one-way ANOVA while Receiver operating characteristics (ROC) and logistic regression models were used to explore FFA significant for diagnosing NAFLD. Results NAFLD patients presented significantly higher (P < 0.05) serum FFA profiles compared to healthy controls (HC). While total FFA profiles were insignificantly different between lean (2093.33 [+ or -] 558.11 [mu]g/ml) and overweight (2420.81 [+ or -] 555.18 [mu]g/ml) NAFLD patients, obese NAFLD (2739.01 [+ or -] 810.35 [mu]g/ml) presented most significantly elevated (P < 0.05) total FFA profiles compared with HC. Of the four FFA; myristic acid (14:0), palmitoleic acid (16:1), [gamma]-linolenic acid ([gamma]-18:3) and cis-7,10,13,16,19-docosapentaenoic acid (22:5), selected in ROC analysis given their high Youden's index and AUC, only 14:0; 5.58(1.37, 22.76) and 16:1; 4.36(1.34, 14.13) had statistical significant odd ratios. Conclusion Our findings suggest 14:0 and 16:1 are promising for early diagnosis of NAFLD. Keywords: FFA, Body weight, NAFLD, GC-MS, Myristic acid, Palmitoleic acid
ISSN:1476-511X
1476-511X
DOI:10.1186/s12944-017-0551-1