The response of Phyllophaga brevidens and Phyllophaga lenis benzoate and light

A sexual attractant of some Phyllophaga species (Coleoptera: Scarabaeidae: Melolonthinae), methyl 2-(methylthio)benzoate, was studied alone and in combination with light from a solar-powered light-emitting diode (LED) to determine their comparative attraction potential for Phyllophaga species in a s...

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Bibliographic Details
Published in:The Florida entomologist 2017-09, Vol.100 (3), p.546
Main Authors: Zaragoza-Ortega, Monica, Segura-Leon, Obdulia L, Hernandez-Cruz, Julian, Valdez-Carrasco, Jorge, Saul, Sanchez-Soto
Format: Article
Language:English
Subjects:
Beetles
Insect pheromones
Insect traps
Observations
Sexual behavior
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Summary:A sexual attractant of some Phyllophaga species (Coleoptera: Scarabaeidae: Melolonthinae), methyl 2-(methylthio)benzoate, was studied alone and in combination with light from a solar-powered light-emitting diode (LED) to determine their comparative attraction potential for Phyllophaga species in a sugarcane crop. Four trap treatments were evaluated: sex attractant, sex attractant plus light, light alone, and a control trap without attractant or light. The number and species of Phyllophaga captured were determined over two 5 mo adult emergence periods from Mar-Jul in 2014 and 2015. Additionally, the cytochrome oxidase I (CO1) gene of the species that responded to the compound was amplified, and a phylogenetic tree was constructed using those sequences and sequences found in GenBank. Two species of Phyllophaga, Phyllophaga brevidens (Bates) (Coleoptera: Scarabaeidae) and Phyllophaga lenis (Horn) (Coleoptera: Scarabaeidae), responded to the compound. The traps with sex attractant plus light treatment captured significantly more beetles than the other treatments. Our results suggest that the combination of methyl 2-(methylthio)benzoate and light can be a relatively selective method for the capture of the males of these species of agricultural importance, and it also attracts some females. Finally, analysis of the sequences of the mitochondrial CO1 gene showed that the Phyllophaga species captured with this compound were phylogenetically related. Key Words: solar cell; LED; June beetles; May beetles; sugarcane Se evaluo 2-(metiltio)benzoato de metilo, un atrayente sexual, de algunas especies de Phyllophaga (Coleoptera: Scarabaeidae: Melolonthinae), solo y en combinacion con iluminacion de un diodo emisor de luz (LED) impulsado por energia solar, para determinar su capacidad de atraccion comparativa para especies de Phyllophaga dentro del cultivo de cafia de azucar. Se evaluaron cuatro tratamientos de trampas: un atrayente sexual, un atrayente sexual mas luz LED, solo luz y una trampa de control sin atrayente ni luz. Se determino el numero de especies de Phyllophaga capturados por trampa en dos periodos de emergencia de los adultos, de marzo a julio del 2014 y 2015. Tambien, se amplifico el gen CO1 de las especies que respondieron al compuesto y se reconstruyo un arbol filogenetico utilizando estas secuencias y secuencias encontradas en el banco de genes. Dos especies del genero Phyllophaga, P. brevidens (Bates) y P. lenis (Horn) respondieron al compuesto. Las t
ISSN:0015-4040