Nutrient Stimulation of Indigenous Microorganisms for Oil-in-Water Emulsion in a Medium Temperature Petroleum Reservoir with Ca[sup.2+]-Rich Brine

One of the challenges indigenous microbial enhanced oil recovery (MEOR) is facing is the high percentage of divalent ions, which obstruct the growth and metabolism of microorganisms and destabilize the oil-in-water (o/w) emulsion. Six formulas were selected for the stimulation of indigenous microbes...

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Bibliographic Details
Published in:Geofluids 2021, Vol.2021
Main Authors: Shi, Guoxin, Jia, Yuanyuan, Bai, Lei, Li, Guoqiang, Liu, Xiaoli, Liu, Meichen, Dai, Xuecheng
Format: Article
Language:English
Subjects:
Microorganisms
Oil fields
Petroleum mining
RNA
Online Access:Get full text
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Summary:One of the challenges indigenous microbial enhanced oil recovery (MEOR) is facing is the high percentage of divalent ions, which obstruct the growth and metabolism of microorganisms and destabilize the oil-in-water (o/w) emulsion. Six formulas were selected for the stimulation of indigenous microbes and to compare their performances on the oil emulsification and oil spreading in the Luliang oilfield containing Ca[sup.2+]-rich brine. Illumina MiSeq sequencing of 16S rRNA genes was applied to investigate the structural response of microbial communities to various formulas. The results showed that the addition of proper organic phosphorus and the optimal P/N ratio (0.01) can facilitate production of biosurfactant and create stable o/w emulsion with specific reservoir condition containing Ca[sup.2+]-rich brine, through direct stimulation of certain functional microbes. This study provides a promising path for direct enrichment of biosurfactant-producing and oil-degrading Dietzia genus and a potential instructional approach of indigenous MEOR in Luliang oilfield.
ISSN:1468-8115
DOI:10.1155/2021/6659038