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Occurrence of Pyricularia oryzae Triticum in plants of the genus Urochloa in Brazil/Ocorrencia de Pyricularia oryzae Triticum em plantas do genero Urochloa no Brasil

In this study Pyricularia spp., P. oryzae and the P. oryzae pathotype Triticum (PoT) were detected and identified in leaf segments of forage and invasive grasses located in or next to wheat fields. In 2018 and 2019, 66 samples of lesion leaf segments of Urochloa and other grasses were collected in L...

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Bibliographic Details
Published in:Ciência rural 2023-04, Vol.53 (4), p.1
Main Authors: Maciel, Joao Leodato Nunes, Kovaleski, Marcos, Silva, Alieze Nascimento da, Bonato, Ana Lidia Variani, Costa, Ivan Francisco Dressler da
Format: Article
Language:Portuguese
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Summary:In this study Pyricularia spp., P. oryzae and the P. oryzae pathotype Triticum (PoT) were detected and identified in leaf segments of forage and invasive grasses located in or next to wheat fields. In 2018 and 2019, 66 samples of lesion leaf segments of Urochloa and other grasses were collected in Londrina (PR), Patos de Minas (MG), and Uberaba (MG). The detection and/or identification of the pathogens on the samples was conducted using moist chamber procedures and with the primers MoT3 and Pot2 by PCR. There were DNA amplification with the primer MoT3 (specific for PoT) for 13 (19.69%) of the samples, all of them from Urochloa. The finding that Urochloa hosts PoT at a relatively high rate raises concerns about the importance which these plants may have on the wheat blast cycle as an alternative host for the pathogen and/or source of inoculum for the disease. Key words: wheat blast, primer MoT3, source of inoculum. Neste estudo Pyricularia spp., P. oryzae e o patotipo Triticum (PoT) de P. oryzae foram detectados e identificados em segmentos foliares de forrageiras e gramineas invasoras de lavouras de trigo. Em 2018 e 2019, foram coletadas 66 amostras de segmentos foliares lesionados de Urochloa e outras gramineas em Londrina (PR), Patos de Minas (MG) e Uberaba (MG). A deteccao e/ou identificacao dos patogenos nas amostras foi realizada por meio de procedimentos de camara umida e com os iniciadores MoT3 e Pot2 por PCR. Houve amplificacoes de DNA com o primer MoT3 (especifico para PoT) em 13 (19,69%) das amostras, todas provenientes da Urochloa. O resultado de que Urochloa hospeda PoT em uma taxa relativamente alta levanta preocupacoes sobre a importancia que essas plantas podem ter no ciclo de brusone do trigo como hospedeiro intermediario para o patogeno e / ou fonte de inoculo para a doenca. Palavras-chave: brusone do trigo, primer de PCR MoT3, fonte de inoculo.
ISSN:0103-8478
DOI:10.1590/0103-8478cr20210839