Sandwich-type microRNA biosensor based on graphene oxide incorporated 3D-flower-like MoS.sub.2 and AuNPs coupling with HRP enzyme signal amplification

A sandwich electrochemical biosensing strategy for ultrasensitive detection of miRNA-21 was developed by using graphene oxide incorporated 3D-flower-like MoS.sub.2 (3D MoS.sub.2-rGO) nanocomposites as the substrate and horseradish peroxidase (HRP)-functionalized DNA strand 1 (S1)-gold nanoparticles...

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Bibliographic Details
Published in:Mikrochimica acta (1966) 2022-01, Vol.189 (1)
Main Authors: Dong, Jiangbo, Yang, Huisi, Zhao, Jiaying, Wen, Li, He, Congjuan, Hu, Zhikun, Li, Jiawei
Format: Article
Language:English
Subjects:
Analysis
Detectors
Electric properties
Enzymes
Ethylenediaminetetraacetic acid
Graphene
Graphite
MicroRNA
Online Access:Get full text
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Summary:A sandwich electrochemical biosensing strategy for ultrasensitive detection of miRNA-21 was developed by using graphene oxide incorporated 3D-flower-like MoS.sub.2 (3D MoS.sub.2-rGO) nanocomposites as the substrate and horseradish peroxidase (HRP)-functionalized DNA strand 1 (S1)-gold nanoparticles (S1-AuNPs-HRP) as signal amplification probes. Herein, 3D MoS.sub.2-rGO nanocomposites not only had a large specific surface area and excellent conductivity, but also provided more attachment sites for electrodepositing AuNPs. In the presence of target miRNA, a sandwich structure was formed, and the determination of the miRNA-21 was carried out by measuring the DPV response of H.sub.2O.sub.2 mediated by hydroquinone (HQ) at a potential of + 0.052 V (vs AgCl reference electrode). Under the optimal experimental conditions, the as-prepared biosensor enabled the ultrasensitive detection of miRNA-21 from 5 fM to 0.5 [mu]M with the low detection limit of 0.54 fM (S/N = 3), comparable or lower than previous reported methods for miRNA-21 detection, which benefited from the synergistic amplification of 3D MoS.sub.2-rGO and AuNPs-HRP. The prepared biosensor showed satisfactory selectivity, reproducibility, and stability towards miRNA-21 detection. The biosensor was feasible for accurate and quantitative detection of miRNA-21 in normal human serum samples with RSD below 5.86%, which showed a great potential in clinical analysis and disease diagnosis. Graphical abstract
ISSN:0026-3672
DOI:10.1007/s00604-021-05141-0