Simple amperometric detector for microchip capillary electrophoresis, and its application to the analysis of dopamine and catechol

• Published in: Mikrochimica acta (1966) 2011-07, Vol.174 (1-2), p.123-130
• Main Authors: Li, Xinchun, Pan, Jianbin, Yang, Fan, Feng, Jun, Mo, Jinyuan, Chen, Zuanguang
• Format: Article
• Language: English
• Subjects: Analytical Chemistry; Catechin; Characterization and Evaluation of Materials; Chemistry; Chemistry and Materials Science; Chromatographic methods and physical methods associated with chromatography; Detectors; Electrochemical methods; Electrophoresis; Exact sciences and technology; General, instrumentation; Integrated circuits; Microengineering; Nanochemistry; Nanotechnology; Original Paper; Other chromatographic methods; Semiconductor chips
• ISSN: 0026-3672; 1436-5073
• DOI: 10.1007/s00604-011-0592-5

We report on a simple amperometric detector for use in microchip capillary electrophoresis. A disposable syringe serves as the electrode holder that is fixed at the outlet of the separation channel. A carbon paste electrode is used to detect dopamine (DA) and catechol (CA) after electrophoretic separation. The two model analytes were well separated within 60 s. The response is linear in the concentration range from 4 to 500 μM, and the detection limit is 1.2 μM for DA (S/N = 3:1). The relative standard deviations of the inter-run and inter-electrode peak currents, respectively, are 2.8% and 5.7% for DA, and 3.9% and 6.5% for CA. Favorable column efficiency (expressed by the theoretical plate number which is 5.3 × 104 m -1 for DA) is achieved. The method was successfully applied to the separation and detection of 3-aminophenol (3-AP) in an injection powder containing sodium 4-aminosalicylate. The detection limit of 3-AP is as low as 1.7 μM, which meets the demand of the impurity test. The facile assembly allows convenient replacement of working electrodes and improves the longevity of the microanalytical system. Figure Voltammetric behaviors of 3-AP and 4-AS at carbon paste electrode (CPE), and microchip electrophoresis-amperometry with CPE for the two analytes.