Genome-Wide In Silico Identification, Structural Analysis, Promoter Analysis, and Expression Profiling of IPHT/I Gene Family in Sugarcane Root under Salinity Stress

The phosphate transporter (PHT) family of proteins plays an imperative role in regulating phosphorus (P) acquisition as well as in translocation from the soil into cells and organs. Phosphorus is an essential macronutrient required for plant life that is not readily available to crops, and resources...

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Bibliographic Details
Published in:Sustainability 2022-11, Vol.14 (23)
Main Authors: Murugan, Naveenarani, Palanisamy, Vignesh, Channappa, Mahadevaiah, Ramanathan, Valarmathi, Ramaswamy, Manimekalai, Govindakurup, Hemaprabha, Chinnaswamy, Appunu
Format: Article
Language:English
Subjects:
Environmental aspects
Gene expression
Genetic aspects
Genome-wide association studies
Identification and classification
Physiological aspects
Roots (Botany)
Salt stress (Botany)
Sugarcane
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Summary:The phosphate transporter (PHT) family of proteins plays an imperative role in regulating phosphorus (P) acquisition as well as in translocation from the soil into cells and organs. Phosphorus is an essential macronutrient required for plant life that is not readily available to crops, and resources are diminishing rapidly because of the huge needs of global agriculture. In this study, 23 ShPHT genes were identified in the sugarcane (Saccharum spp.) genome through a comprehensive genome-wide in silico analysis. Phylogeny, gene structure, and conserved motif analysis of PHT genes in sugarcane (ShPHTs) indicated five subfamilies (PHT1-4 and PHO1 subfamily). Gene ontology (GO) analysis revealed that the ShPHT genes were largely involved in phosphate ion transport, phosphate starvation, stimulus response, stress response, and symporter activity. Gene expression analysis under salinity stress confirmed strong induction of PHT genes in wild genotype sugarcane (IND99-907). PHT1-1, PHT1-2, and PHT1-3 members were notably up-regulated in roots under salt stress. The upstream region of PHT genes contained PHR1-binding sites (P1BS), MYB-type, and WRKY- type binding elements. Overall, the present study paves the way for a deeper understanding of the evolution of sugarcane PHT genes and their role in salinity and Pi stress tolerance in sugarcane.
ISSN:2071-1050
2071-1050
DOI:10.3390/su142315893