New Sesquiterpene Glycosides from the Flowers of IAster koraiensis/I and Their Inhibition Activities on EGF- and TPA-Induced Cell Transformation

• Published in: Plants 2023, Vol.12 (8)
• Main Authors: Seo, Young-Hye, Kim, Ji-Young, Ryu, Seung-Mok, Hwang, Sun-Young, Lee, Mee-Hyun, Kim, Nahyun, Son, Hojun, Lee, A-Yeong, Kim, Hyo-Seon, Moon, Byeong-Cheol, Jang, Dae-Sik, Lee, Jun
• Format: Report
• Language: English
• Subjects: Botanical research; Chromatography; Compositae; Epidermal growth factor; Genetic aspects; Physiological aspects; Sesquiterpenes; Spectra; Structure
• ISSN: 2223-7747; 2223-7747
• DOI: 10.3390/plants12081726

In total, four new eudesmane-type sesquiterpene glycosides, askoseosides A–D (1–4), and 18 known compounds (5–22) were isolated from the flowers of Aster koraiensis via chromatographic techniques. Chemical structures of the isolated compounds were identified by spectroscopic/spectrometric methods, including NMR and HRESIMS, and the absolute configuration of the new compounds (1 and 2) was performed by electronic circular dichroism (ECD) studies. Further, the anticancer activities of the isolated compounds (1–22) were evaluated using the epidermal growth factor (EGF)-induced as well as the 12-O-tetradecanoylphorbol 13-acetate (TPA)-induced cell transformation assay. Among the 22 compounds, compounds 4, 9, 11, 13–15, 17, 18, and 22 significantly inhibited both EGF- and TPA-induced colony growth. In particular, askoseoside D (4, EGF: 57.8%; TPA: 67.1%), apigenin (9, EGF: 88.6%; TPA: 80.2%), apigenin-7-O-β-d-glucuronopyranoside (14, EGF: 79.2%; TPA: 70.7%), and 1-(3′,4′-dihydroxycinnamoyl) cyclopentane-2,3-diol (22, EGF: 60.0%; TPA: 72.1%) showed higher potent activities.