Functional Study and Efficient Catalytic Element Mining of CYP76AHs in ISalvia/I Plants

Salvia is a large genus with hundreds of species used in traditional Chinese medicine. Tanshinones are a highly representative class of exclusive compounds found in the Salvia genus that exhibit significant biological activity. Tanshinone components have been identified in 16 Salvia species. The CYP...

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Bibliographic Details
Published in:Molecules (Basel, Switzerland) Switzerland), 2023-06, Vol.28 (12)
Main Authors: Zhao, Zhenyu, Yang, Dongfeng, Guo, Juan, Liu, Xiuyu, Li, Qishuang, Su, Ping, Wang, Jian, Ma, Ying, Huang, Luqi
Format: Article
Language:English
Subjects:
Analysis
Evolution
Evolutionary biology
Phylogeny
Plants
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Summary:Salvia is a large genus with hundreds of species used in traditional Chinese medicine. Tanshinones are a highly representative class of exclusive compounds found in the Salvia genus that exhibit significant biological activity. Tanshinone components have been identified in 16 Salvia species. The CYP76AH subfamily (P450) is crucial for the synthesis of tanshinone due to its catalytic generation of polyhydroxy structures. In this study, a total of 420 CYP76AH genes were obtained, and phylogenetic analysis showed their clear clustering relationships. Fifteen CYP76AH genes from 10 Salvia species were cloned and studied from the perspectives of evolution and catalytic efficiency. Three CYP76AHs with significantly improved catalytic efficiency compared to SmCYP76AH3 were identified, providing efficient catalytic elements for the synthetic biological production of tanshinones. A structure–function relationship study revealed several conserved residues that might be related to the function of CYP76AHs and provided a new mutation direction for the study of the directed evolution of plant P450.
ISSN:1420-3049
1420-3049
DOI:10.3390/molecules28124711