Safety and Immunogenicity of Chimeric IPestivirus/I KD26_E2LOM in Piglets and Calves

A chimeric pestivirus (KD26_E2LOM) was prepared by inserting the E2 gene of the classical swine fever virus (CSFV) LOM strain into the backbone of the bovine viral diarrhea virus (BVDV) KD26 strain. KD26_E2LOM was obtained by transfecting the cDNA pACKD26_E2LOM into PK-15 cells. KD26_E2LOM chimeric...

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Bibliographic Details
Published in:Vaccines 2023, Vol.11 (10)
Main Authors: Park, Gyu-Nam, Shin, Jihye, Choe, SeEun, Kim, Ki-Sun, Kim, Jae-Jo, Lim, Seong-In, An, Byung-Hyun, Hyun, Bang-Hun, An, Dong-Jun
Format: Report
Language:English
Subjects:
Animals
Cattle
Drug therapy
Infancy
Prevention
RNA virus infections
Swine
Testing
Viral vaccines
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Summary:A chimeric pestivirus (KD26_E2LOM) was prepared by inserting the E2 gene of the classical swine fever virus (CSFV) LOM strain into the backbone of the bovine viral diarrhea virus (BVDV) KD26 strain. KD26_E2LOM was obtained by transfecting the cDNA pACKD26_E2LOM into PK-15 cells. KD26_E2LOM chimeric pestivirus proliferated to titers of 10[sup.6.5] TCID[sub.50] /mL and 10[sup.8.0] TCID[sub.50] /mL at 96 h post-inoculation into PK-15 cells or MDBK cells, respectively. It also reacted with antibodies specific for CSFV E2 and BVDV E[sup.rns] , but not with an anti-BVDV E2 antibody. Piglets (55–60 days old) inoculated with a high dose (10[sup.7.0] TCID[sub.50] /mL) of KD26_E2LOM produced high levels of CSFV E2 antibodies. In addition, no co-habiting pigs were infected with KD26_E2LOM; however, some inoculated pigs excreted the virus, and the virus was detected in some organs. When pregnant sows were inoculated during the first trimester (55–60 days) with a high dose (10[sup.7.0] TCID[sub.50] /mL) of KD26_E2LOM, anti-CSFV E2 antibodies were produced at high levels; chimeric pestivirus was detected in one fetus and in the ileum of one sow. When 5-day-old calves that did not consume colostrum received a high dose (10[sup.7.0] TCID[sub.50] /mL) of KD26_E2LOM, one calf secreted the virus in both feces and nasal fluid on Day 2. A high dose of KD26_E2LOM does not induce specific clinical signs in most animals, does not spread from animal to animal, and generates CSFV E2 antibodies with DVIA functions. Therefore, chimeric pestivirus KD26_E2LOM is a potential CSFV live marker vaccine.
ISSN:2076-393X
2076-393X
DOI:10.3390/vaccines11101622