Profiling of Volatile Compounds in ‘Muscat Hamburg’ Contaminated with IAspergillus carbonarius/I before OTA Biosynthesis Based on HS-SPME-GC-MS and DLLME-GC-MS

Aspergillus carbonarius is known to produce the carcinogenic ochratoxin A (OTA) in grapes. The metabolism process before OTA biosynthesis influences the content and composition of the volatile compounds in grapes. In this study, a self-established method based on QuEChERS coupled with high-performan...

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Bibliographic Details
Published in:Molecules (Basel, Switzerland) Switzerland), 2024-01, Vol.29 (3)
Main Authors: Guo, Yayun, Wang, Zhe, He, Yi, Gao, Huanhuan, Shi, Hongmei
Format: Article
Language:English
Subjects:
Analysis
Biosynthesis
Contamination
Esters
Grapes
High performance liquid chromatography
Information management
Instrument industry
Mass spectrometry
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Summary:Aspergillus carbonarius is known to produce the carcinogenic ochratoxin A (OTA) in grapes. The metabolism process before OTA biosynthesis influences the content and composition of the volatile compounds in grapes. In this study, a self-established method based on QuEChERS coupled with high-performance liquid chromatography–fluorescence detection (HPLC-FLD) was used to determine the OTA levels during a seven-day contamination period. The results showed that OTA was detected on the second day after contamination with A. carbonarius. Thus, the first day was considered as the critical sampling timepoint for analyzing the volatiles in grapes before OTA biosynthesis. Additionally, the volatile compounds in grapes were analyzed using headspace solid-phase microextraction gas chromatography–mass spectrometry (HS-SPME-GC-MS) and dispersive liquid–liquid microextraction gas chromatography–mass spectrometry (DLLME-GC-MS). The corresponding data were evaluated via multivariate data analysis using projection methods, including PCA and OPLS-DA. The results indicated significant differences in the nine volatile compounds in grapes contaminated with A. carbonarius before OTA biosynthesis. The results of the Pearson correlation analysis showed positive correlations between ethyl acetate, styrene, 1-hexanol and OTA; (E)-2-hexenal and nerolic acid were negatively correlated with OTA. Overall, these findings provide a theoretical basis for the early prediction of OTA formation in grape and grape products using GC-MS technology.
ISSN:1420-3049
1420-3049
DOI:10.3390/molecules29030567