Identification of the Trace Components in BopuzongJian and IMacleaya cordata/I Extract Using LC-MS Combined with a Screening Method

• Published in: Molecules (Basel, Switzerland) Switzerland), 2021-07, Vol.26 (13)
• Main Authors: Dong, Zhuang, Liu, Mengting, Zhong, Xiaohong, Ou, Xiaoyong, Yun, Xuan, Wang, Mingcan, Ren, Shurui, Qing, Zhixing, Zeng, Jianguo
• Format: Article
• Language: English
• Subjects: High performance liquid chromatography; Methods
• ISSN: 1420-3049; 1420-3049
• DOI: 10.3390/molecules26133851

BopuzongJian and Macleaya cordata extract were developed from Macleayacordata and are widely used in agriculture and animal husbandry, but their trace components have been rarely reported in the literature. Trace component analysis is of great importance to the quality and safety of veterinary drugs. In this study, high-performance liquid chromatography/quadrupole time-of-flight mass spectrometry (HPLC-Q-TOF-MS) combined with a screening method was used to screen and characterize the trace components in BopuzongJian and Macleaya cordata extract. A total of 58 trace components were screened from BopuzongJian and Macleaya cordata extract using the screening strategies, of which 39 were identified by their accurate m/z value, characteristic MS/MS data, and fragmentation pathways of references. This established method was used for trace component analysis for the first time and proved to be a useful and rapid tool to screen and identify the trace components of BopuzongJian and Macleaya cordata extract, especially for those at trace levels in a complex sample. In addition, this study marks the first comprehensive research into trace components in these two products and has great significance for the systematic detection of trace components in other plant-derived drugs.