GENETIC ANALYSIS OF CALMODULIN AND ITS TARGETS IN SACCHAROMYCES CEREVISIAE

Calmodulin, a small, ubiquitous Ca 2+ -binding protein, regulates a wide variety of proteins and processes in all eukaryotes. CMD1 , the single gene encoding calmodulin in S. cerevisiae , is essential, and this review discusses studies that identified many of calmodulin's physiological targets...

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Bibliographic Details
Published in:Annual review of genetics 2001-01, Vol.35 (1), p.647-672
Main Author: Cyert, Martha S
Format: Article
Language:English
Subjects:
Analysis
Binding Sites
Calcineurin
calcium
Calmodulin
Calmodulin - genetics
Calmodulin - metabolism
CMD1 gene
Cmk1 protein
Cmk2 protein
DNA, Fungal - genetics
Fungal Proteins - genetics
Fungal Proteins - metabolism
Genes
Genetic aspects
Genetic research
Genetics
Mitosis - genetics
Mitosis - physiology
Myo2 protein
Myosin
Nuf1 protein
Saccharomyces cerevisiae
Saccharomyces cerevisiae - genetics
Saccharomyces cerevisiae - metabolism
signal transduction
spindle pole body
Sps110 protein
Transduction
Yeast
Yeasts
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Summary:Calmodulin, a small, ubiquitous Ca 2+ -binding protein, regulates a wide variety of proteins and processes in all eukaryotes. CMD1 , the single gene encoding calmodulin in S. cerevisiae , is essential, and this review discusses studies that identified many of calmodulin's physiological targets and their functions in yeast cells. Calmodulin performs essential roles in mitosis, through its regulation of Nuf1p/Spc110p, a component of the spindle pole body, and in bud growth, by binding Myo2p, an unconventional class V myosin required for polarized secretion. Surprisingly, mutant calmodulins that fail to bind Ca 2+ can perform these essential functions. Calmodulin is also required for endocytosis in yeast and participates in Ca 2+ -dependent, stress-activated signaling pathways through its regulation of a protein phosphatase, calcineurin, and the protein kinases, Cmk1p and Cmk2p. Thus, calmodulin performs important physiological functions in yeast cells in both its Ca 2+ -bound and Ca 2+ -free form.
ISSN:0066-4197
1545-2948
DOI:10.1146/annurev.genet.35.102401.091302